A8273
抗 α-淀粉酶 兔抗
fractionated antiserum, lyophilized powder
别名:
Alpha Amylase Antibody, Alpha Amylase Antibody - Anti-α-Amylase antibody produced in rabbit
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i), ODD
Citations:
111
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
fractionated antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
packaging
vial of 2 mL lyophilized antiserum
technique(s)
Ouchterlony double diffusion: suitable, indirect ELISA: 1:4,000-1:6,000
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... AMY1A(276)
General description
α-淀粉酶主要在唾液腺和胰腺中大量产生。在空肠和乳腺中也可表达。编码α淀粉酶的基因是位于1号染色体短臂上的AMY1和AMY2。AMY1负责在唾液和乳腺中产生这种酶，而AMY2则在胰腺中合成这种酶。AMY1也在某些肿瘤组织中表达。哺乳动物淀粉酶由三个结构域（A、B和C）组成。结构域A包含具有两个天冬氨酸和一个谷氨酸残基的活性位点。结构域B毗邻活性位点区，它是维持蛋白质构象是必不可少的结构。结构域C涉及催化机理。
Immunogen
人唾液α-淀粉酶。
Application
兔源抗α-淀粉酶抗体可用于免疫荧光和免疫组织化学分析。
兔源抗α淀粉酶抗体还被用于免疫细胞化学分析。
将小鼠胰腺切片固定在4％多聚甲醛中，并使用兔抗淀粉酶抗体以1：100的稀释度用于免疫组织化学。
Biochem/physiol Actions
α--淀粉酶对于催化淀粉消化的第一步是必不可少的，淀粉消化是人类饮食中碳水化合物的主要来源。α-淀粉酶水解多糖，最终生成的主要产物为麦芽糖、麦芽三糖和极限糊精。
该抗血清对在人唾液和人胰腺提取物中发现的人α-淀粉酶具有特异性。未观察到与其他人唾液蛋白或胰腺提取蛋白的反应。
Physical form
由 0.01M 的磷酸缓冲盐溶液 (pH 7.2) 冻干
Preparation Note
用 2 mL 去离子水复溶。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Human alpha-amylase and starch digestion: An interesting marriage
Butterworth PJ, et al.
Starch/Staerke, 63(7), 395-405 (2011)
Maho Kodama et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 56(1), 33-44 (2007-09-19)
In this study, we describe pancreatic cell ontogeny in renal capsule-transplanted embryonic stem cells (ES) after injury by streptozocin (STZ), showing pancreatogenesis in situ. Seven-week-old female BALB/c nude mice were treated with either a single 175- or 200-mg/kg STZ dose
Angela Criscimanna et al.
Gastroenterology, 147(5), 1106-1118 (2014-08-17)
Although the cells that contribute to pancreatic regeneration have been widely studied, little is known about the mediators of this process. During tissue regeneration, infiltrating macrophages debride the site of injury and coordinate the repair response. We investigated the role
Imane Song et al.
PloS one, 10(10), e0140148-e0140148 (2015-10-10)
One week of treatment with EGF and gastrin (EGF/G) was shown to restore normoglycemia and to induce islet regeneration in mice treated with the diabetogenic agent alloxan. The mechanisms underlying this regeneration are not fully understood. We performed genetic lineage
Ultrastructural analysis of pancreatic acinar cells from mice fed on genetically modified soybean
Malatesta M, et al.
Journal of Anatomy, 201(5), 409-415 (2002)
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A8273-1VL | 04061833391372 |
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