生物来源
mouse
偶联物
CY3 conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M2, monoclonal
表单
buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
种属反应性
all
浓度
~1 mg/mL
技术
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
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一般描述
单克隆抗标记 M2-Cy3(小鼠IgG)抗体与花菁染料 Cy3 共价偶联。抗体缀合物结合FLAG融合蛋白,并且将在FLAG融合蛋白的N末端、Met-N末端或C末端以识别FLAG序列。
应用
此抗体已成功使用的应用,以及相关的同行评审论文如下。
蛋白免疫分析(Western Blotting)(1篇文章)
蛋白免疫分析(Western Blotting)(1篇文章)
外形
溶于磷酸盐缓冲盐水,含有 1%BSA 和防腐剂
制备说明
用 Tris 缓冲盐水 (TBS) 稀释抗体:0.05 MTris,pH 7.4,含 0.15 M NaCl。
其他说明
免疫细胞化学的建议浓度为1-10 mg / ml。
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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