B0561
Monoclonal Anti-BUB1 antibody produced in mouse
2 mg/mL, clone 14H5, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Budding Uninhibited By Benzimidazoles 1
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关于此项目
Conjugate:
unconjugated
Clone:
14H5, monoclonal
Application:
ARR, ELISA (i), ICC, IP, WB
Citations:
6
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
14H5, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
2 mg/mL
technique(s)
immunocytochemistry: 4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BUB1(699)
General description
BUB1 is spindle checkpoint kinase that modulates kinetochore-independent segregation of chromosomes and cell proliferation. This kinase regulates the phosphorylation of MAD1 and itself. As a result, BUB1 mediates chromosome-spindle attachments during mitosis. BUB1 also phosphorylates Cdc20 which subsequently facilitates accurate checkpoint signaling . Monoclonal Anti-BUB1 antibody is specific for human BUB1 (approx. 150 kDa).
Immunogen
recombinant human BUB1.
Application
Monoclonal Anti-BUB1 antibody is suitable for use in western blot, indirect ELISA, microarray, immunocytochemistry (4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol) and immunoprecipitation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Zhanyun Tang et al.
Molecular cell, 16(3), 387-397 (2004-11-05)
To ensure the fidelity of chromosome segregation, the spindle checkpoint blocks the ubiquitin ligase activity of APC/C(Cdc20) in response to a single chromatid not properly attached to the mitotic spindle. Here we show that HeLa cells depleted for Bub1 by
T W Seeley et al.
Biochemical and biophysical research communications, 257(2), 589-595 (1999-04-13)
The BUB/MAD signaling pathway monitors attachment of chromosomes to spindle poles in mitotic cells. Mutations of the human BUB1 locus were identified in cancer cells exhibiting an unstable chromosomal complement. We report that the human BUB3 gene maps to a
B Ouyang et al.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, 9(10), 877-885 (1998-10-28)
Eukaryotic cells have evolved a mechanism that delays the onset of anaphase until chromosomes are properly positioned on the spindle. To understand the molecular basis of such surveillance mechanism in human cells, we have cloned a full-length cDNA encoding a
Min Wu et al.
Nature communications, 10(1), 273-273 (2019-01-19)
Faithful chromosome segregation requires proper chromosome congression at prometaphase and dynamic maintenance of the aligned chromosomes at metaphase. Chromosome missegregation can result in aneuploidy, birth defects and cancer. The kinetochore-bound KMN network and the kinesin motor CENP-E are critical for
Christiane Klebig et al.
The Journal of cell biology, 185(5), 841-858 (2009-06-03)
The kinetochore-bound protein kinase Bub1 performs two crucial functions during mitosis: it is essential for spindle checkpoint signaling and for correct chromosome alignment. Interestingly, Bub1 mutations are found in cancer tissues and cancer cell lines. Using an isogenic RNA interference
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