等级
Molecular Biology
描述
T1R
表单
suspension
运输
dry ice
储存温度
−70°C
一般描述
BL21-T1R are competent E. coli that are suitable for high level expression/production of heterologous proteins regulated by various expression vector systems. The cells have transformation efficiency of ≥3x106 cfu/μg when transformed with non-saturating amounts of pUC19 plasmid DNA.
Sigma′s BL21-T1R competent E. coli cells are grown and made chemically competent using an optimized procedure specific to the strain, followed by strain verification and efficiency testing. The cells are provided in frozen 50 μl aliquots for convenience. Each aliquot can be used for a single transformation.
应用
Suitable for expression and production of proteins directed by a range of expression systems with promoters such as lac, trc, tac, λPL and araD
生化/生理作用
BL21-T1R does not express ion proteases and outer membrane protease, ompT. This prevents the degradation of heterologous proteins expressed by various expression vector systems. This strain also contains tonA genotype that confers resistance to lytic bacteriophages such as T1 and T5.
特点和优势
- Ensures recovery of heterologous proteins
- Contains the genotype tonA that protects the clonal stocks from lytic bacteriophages
- Guaranteed high transformation efficiency
- Convenient 50 μL aliquots
其他说明
- BL21-T1R chemically competent cells, 10 X 50 μL (B2810)
- pUC 19 control DNA (10 ng/μL), 10 μL (D2567)
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
new publication: Bacterial receptors for phages and colicins as constituents of specific transport system, in Receptors and Recognition, Series B, Vol. 3, J. L. Reissig (Ed.), pp. 101-130. (Chapman & Hall, Ltd., London, 1977).
Braun, V., and Hantke, K.
Receptors and Recognition, 3, 101-130 (1977)
T A Phillips et al.
Journal of bacteriology, 159(1), 283-287 (1984-07-01)
The product of the pleiotropic gene lon is a protein with protease activity and has been tentatively identified as protein H94.0 on the reference two-dimensional gel of Escherichia coli proteins. Purified Lon protease migrated with the prominent cellular protein H94.0
Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.
W B Wood
Journal of molecular biology, 16(1), 118-133 (1966-03-01)
Losee L Ling et al.
Nature, 517(7535), 455-459 (2015-01-07)
Antibiotic resistance is spreading faster than the introduction of new compounds into clinical practice, causing a public health crisis. Most antibiotics were produced by screening soil microorganisms, but this limited resource of cultivable bacteria was overmined by the 1960s. Synthetic
Adam Johnson et al.
The EMBO journal, 34(6), 811-827 (2015-01-15)
In mammalian cells, cargo-laden secretory vesicles leave the endoplasmic reticulum (ER) en route to ER-Golgi intermediate compartments (ERGIC) in a manner dependent on the COPII coat complex. We report here that COPII-coated transport carriers traverse a submicron, TFG (Trk-fused gene)-enriched
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