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Merck
CN

B3111

ANTI-FLAG® M2 antibody, Mouse monoclonal

Clone M2, purified from hybridoma cell culture in bioreactor

别名:

Anti-ddddk, Anti-dykddddk, M2 clone ANTI-FLAG

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关于此项目

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
M2, monoclonal
Species reactivity:
-
Application:
Citations:
19
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biological source

mouse

antibody form

purified immunoglobulin (purified IgG1 subclass)

clone

M2, monoclonal

shelf life

4 yr

purified by

using Protein A

storage temp.

−20°C

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General description

Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.

form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL

Application

IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.

Learn more product details in our FLAG® application portal.

Packaging

polypropylene screw cap vial

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in specified buffer

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料
常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Runming Zeng et al.
Experimental biology and medicine (Maywood, N.J.), 246(6), 644-653 (2020-12-11)
Osteoarthritis (OA), the most prevalent form of arthritis disease, is characterized by destruction of articular cartilage, osteophyte development, and sclerosis of subchondral bone. Transcription factors Janus kinase 1/signal transducer and activator of transcription 3 (JAK1/STAT3) and Forkhead box M1 (FOXM1)
Dongdong Shen et al.
PLoS pathogens, 20(7), e1012256-e1012256 (2024-07-18)
African swine fever (ASF) is a highly contagious, fatal disease of pigs caused by African swine fever virus (ASFV). The complexity of ASFV and our limited understanding of its interactions with the host have constrained the development of ASFV vaccines
Xin Wu et al.
mBio, 13(2), e0040222-e0040222 (2022-03-29)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection triggers cytokine-mediated inflammation, leading to a myriad of clinical presentations in COVID-19. The SARS-CoV-2 open reading frame 8 (ORF8) is a secreted and rapidly evolving glycoprotein. Patients infected with SARS-CoV-2 variants with
Yi-Min Chu et al.
Frontiers in oncology, 12, 900166-900166 (2022-10-04)
DLC1 (deleted in liver cancer-1) is downregulated or deleted in colorectal cancer (CRC) tissues and functions as a potent tumor suppressor, but the underlying molecular mechanism remains elusive. We found that the conditioned medium (CM) collected from DLC1-overexpressed SW1116 cells
Arturo Roca-Rivada et al.
JCI insight, 9(9) (2024-05-08)
The importance of the proper localization of most receptors at the cell surface is often underestimated, although this feature is essential for optimal receptor response. Endospanin 1 (Endo1) (also known as OBRGRP or LEPROT) is a protein generated from the

全球贸易项目编号

货号GTIN
B3111-0.2MG04061841248507
B3111-1MG04061841248514

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