Anti-CSTF1 (N-terminal) antibody produced in rabbit

Cleavage stimulation factor (CSTF) is a heterotrimer consisting of subunits of 50, 64 and 77 kDa referred to as CSTF1, CSTF2 and CSTF3 respectively. Anti-CSTF1 (N-terminal) is produced in rabbit using a synthetic peptide as immunogen. It corresponds to amino acids 2-16 of human CSTF1 conjugated to KLH. The corresponding sequence is identical in mouse and rat. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Anti-CSTF1 is used in immunoprecipitation at a concentration of 5-10 μg using K562 cell lysates. The antibody may be used in several immunochemical techniques including immunoblotting (∼48 kDa).

CSTF1 is required for in vitro cleavage activity. CSTF1 also interacts directly with the RNA polymerase II CTD, suggesting a role in linking transcription and mRNA 3′-ends processing. It is required for polyadenylation and 3′-end cleavage of mammalian pre-mRNAs.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

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