C2872
Anti-CSTF1 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-Cleavage stimulation factor, 3-prime PRE-RNA, subunit 1, 50-KD, Anti-CstF-50, Anti-CstFp50
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~48 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg using K562 cell lysates, western blot: 1-2 μg/mL using HepG2 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CSTF1(1477)
mouse ... Cstf1(67337)
rat ... Cstf1(311670)
General description
Cleavage stimulation factor (CSTF) is a heterotrimer consisting of subunits of 50, 64 and 77 kDa referred to as CSTF1, CSTF2 and CSTF3 respectively. Anti-CSTF1 (N-terminal) is produced in rabbit using a synthetic peptide as immunogen. It corresponds to amino acids 2-16 of human CSTF1 conjugated to KLH. The corresponding sequence is identical in mouse and rat. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
Application
Anti-CSTF1 is used in immunoprecipitation at a concentration of 5-10 μg using K562 cell lysates. The antibody may be used in several immunochemical techniques including immunoblotting (∼48 kDa).
Biochem/physiol Actions
CSTF1 is required for in vitro cleavage activity. CSTF1 also interacts directly with the RNA polymerase II CTD, suggesting a role in linking transcription and mRNA 3′-ends processing. It is required for polyadenylation and 3′-end cleavage of mammalian pre-mRNAs.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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J Zhao et al.
Microbiology and molecular biology reviews : MMBR, 63(2), 405-445 (1999-06-05)
Formation of mRNA 3' ends in eukaryotes requires the interaction of transacting factors with cis-acting signal elements on the RNA precursor by two distinct mechanisms, one for the cleavage of most replication-dependent histone transcripts and the other for cleavage and
C R Mandel et al.
Cellular and molecular life sciences : CMLS, 65(7-8), 1099-1122 (2007-12-26)
Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3'-end. Processing at the 3'-end is controlled by sequence elements in the pre-mRNA (cis elements) as well as protein factors. Despite the seeming biochemical simplicity
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