C3172
肌酐酶 来源于微生物
lyophilized powder, 100-300 units/mg protein
别名:
肌酐酰胺水解酶
产品名称
肌酐酶 来源于微生物, lyophilized powder, 100-300 units/mg protein
form
lyophilized powder
specific activity
100-300 units/mg protein
mol wt
~175 kDa
composition
Protein, 65-85%
foreign activity
Creatinase and urease ≤1%
Hexokinase and ATPase ≤0.1%
storage temp.
2-8°C
Quality Level
Analysis Note
通过缩二脲法测定的蛋白质。
Application
在临床分析中，该酶可与肌酸酶、肌氨酸脱氢酶或肌氨酸氧化酶以及甲醛脱氢酶结合用于酶法测定肌酐。
来自微生物的肌酐酶可通过与其他酶共固定进行肌酐测定而用于安培生物传感器的制备。
Biochem/physiol Actions
来自假单胞菌属的肌酐酶是一种每个亚基分子量为28.4 kDa的同六聚体酶。它是一种环状酰胺水解酶，可催化肌酸酐向肌酸的可逆性转化。每个单体在β-链的C末端和主要α-螺旋的N末端附近都含有一个双核锌中心。这些锌离子指示着活性位点的位置。
General description
等电点： 4.7
米氏常数：3.2 x 10‾2M（肌苷），5.7 x 10‾2M（肌酸）
结构：每摩尔的酶6个亚基（每个亚基结合一摩尔的锌）
抑制剂：Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值： 6.5 − 7.5
最适温度： 70°C
pH稳定性： pH 7.5 − 9.0 (5°C，16小时)
热稳定性： 低于70°C（pH 7.5，30分钟）
米氏常数：3.2 x 10‾2M（肌苷），5.7 x 10‾2M（肌酸）
结构：每摩尔的酶6个亚基（每个亚基结合一摩尔的锌）
抑制剂：Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值： 6.5 − 7.5
最适温度： 70°C
pH稳定性： pH 7.5 − 9.0 (5°C，16小时)
热稳定性： 低于70°C（pH 7.5，30分钟）
Other Notes
在pH 8.0、25 °C条件下，一个单位将在每分钟内水解1.0 μmole的肌苷
Physical form
含有蔗糖和BSA作为稳定剂的冻干粉末
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
含少量动物源组分生物产品
此项目有
Tadashi Yoshimoto et al.
Journal of molecular biology, 337(2), 399-416 (2004-03-09)
Creatininase from Pseudomonas putida is a member of the urease-related amidohydrolase superfamily. The crystal structure of the Mn-activated enzyme has been solved by the single isomorphous replacement method at 1.8A resolution. The structures of the native creatininase and the Mn-activated
Kinuyo Yamashita et al.
Journal of molecular biology, 396(4), 1081-1096 (2010-01-02)
Creatininase is a binuclear zinc enzyme and catalyzes the reversible conversion of creatinine to creatine. It exhibits an open-closed conformational change upon substrate binding, and the differences in the conformations of Tyr121, Trp154, and the loop region containing Trp174 were
Vannajan Sanghiran Lee et al.
Journal of computer-aided molecular design, 24(10), 879-886 (2010-08-31)
The reaction mechanism of creatinine-creatininase binding to form creatine as a final product has been investigated by using a combined ab initio quantum mechanical/molecular mechanical approach and classical molecular dynamics (MD) simulations. In MD simulations, an X-ray crystal structure of
Amperometric creatinine biosensor for hemodialysis patients
Tombach B, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 312(1-2), 129-134 (2001)
Antonino S Rubino et al.
The Annals of thoracic surgery, 91(2), 534-540 (2011-01-25)
Leukocyte filtration has been reported to reduce inflammatory damage during cardiopulmonary bypass. We evaluated the role of leukocyte filtration on hospital outcome and postoperative morbidity. Eighty-two consecutive patients who underwent isolated coronary artery bypass grafting were randomly assigned (1:1) to
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