生物来源
rat
重组
expressed in E. coli
方案
≥98% (SDS-PAGE)
表单
lyophilized powder
分子量
22.7 kDa
包装
pkg of 10 μg
储存条件
avoid repeated freeze/thaw cycles
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
颜色
white
溶解性
water: soluble 0.10 mL, clear to slightly hazy, colorless
UniProt登记号
储存温度
−20°C
基因信息
rat ... Cntf(25707)
生化/生理作用
CNTF prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation; most of its known actions are restricted to cells of the nervous system.
Ciliary neurotrophic factor was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons, and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation. Outside the nervous system, CNTF maintains embryonic stem cells in an undifferentiated, pluripotent state. CNTF is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). CNTF exerts its actions through the activation of the high-affinity CTNF receptor complex, which contains the ligand-binding α-subunit (CNTF Rα) and two signal transducing β-subunits (LIF Rβ and gp130). CNTF is localized in the cell nucleus subsequent to receptor binding. Human and rat ciliary neurotrophic factor share ~83% sequence homology and show cross-reactivity in bioactivity.
外形
Lyophilized from a 0.2 μm filtered solution of 5 mM sodium acetate, pH 6.5.
分析说明
The proliferative activity is tested in a cell proliferation assay using the cytokine-dependent human erythroleukemic cell line, TF-1.
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
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