C4405
胆碱氧化酶 来源于球形节杆菌
lyophilized powder, 8-20 units/mg solid
别名:
胆碱:氧1-氧化还原酶
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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-840-0
MDL number:
Specific activity:
8-20 units/mg solid
产品名称
胆碱氧化酶 来源于球形节杆菌, lyophilized powder, 8-20 units/mg solid
Quality Level
form
lyophilized powder
specific activity
8-20 units/mg solid
mol wt
71 kDa by SDS-PAGE
83 kDa by gel filtration
storage temp.
−20°C
Application
Sigma的胆碱氧化酶用于使用具有电位检测的流动注射分析（FIA）系统对牛奶中的胆碱进行酶法测定。来自节细菌属的胆碱氧化酶适用于制备安培型生物传感器。
来自球形节杆菌的胆碱氧化酶已被用于：
- 酶法测定胆碱（Cho）和磷酸胆碱（P-Cho）。
- 在鞘磷脂试验中通过测量游离胆碱来测定鞘磷脂的浓度
- 用于电化学测量的全组装记录设备的酶涂层
Biochem/physiol Actions
胆碱氧化酶催化胆碱到甘氨酸甜菜碱的四电子氧化反应，其中甜菜碱醛为中间体，分子氧为初级电子受体。该酶还可以甜菜碱醛作为底物。这可研究胆碱转化成醛中间体和甜菜碱醛转化成甘氨酸甜菜碱的反应机理。该酶是分子量约为83,000 Da（凝胶过滤法）或71,000 Da（SDS凝胶电泳法）的黄素蛋白。最佳pH约为pH 7.5，等电点（pI）约为pH 4.5。
General description
研究领域： 细胞信号传导。胆碱氧化酶是一种黄酮类蛋白，属于葡萄糖甲醇胆碱（GMC）氧化还原酶家族。
Other Notes
在37℃、pH 8.0条件下，一单位酶每分钟可形成1 μmM H2O2 ，同时将1 μmM胆碱氧化成甜菜碱醛。注意：在胆碱氧化酶将胆碱转化成甜菜碱过程中，每μM胆碱产生2 μmM H2O2 。
Hazard Classifications
Resp. Sens. 1
signalword
Danger
hcodes
pcodes
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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The Journal of biological chemistry, 278(7), 4932-4942 (2002-12-06)
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