form
buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
mol wt
antigen 20 kDa (p20 subunit), antigen 45 kDa (p45 proenzyme)
species reactivity
rat, human, mouse
technique(s)
immunocytochemistry: 10 μg/mL using human HL-60 cells, western blot: 0.5-2 μg/mL using human HL-60 cell lysate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CASP1(834)
mouse ... Casp1(12362)
rat ... Casp1(25166)
Immunogen
synthetic peptide corresponding to amino acid residues 129-152 of human caspase 1 conjugated to KLH.
Application
Anti-Caspase 1 (ICE) antibody produced in rabbit has been used in immunoblot assay to analyse CASP-3 and -7 cleavage of WT and DKO cells treated with etoposide. It is suitable for immunocytochemistry at a concentration of 10μg/mL using human HL-60 cells. It is also suitable for western blot at a concentration of 0.5-2μg/mL using human HL-60 cell lysate.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
ICE1 is a MYC-like basic helix-loop-helix transcription factor. It is an upstream activator of the cold-induced transcriptome. ICE1 belongs to the bHLH subfamily 9. It specifically binds to the MYC recognition sites (5′-CANNTG-3′) on the CBF3/DREB1A promoter. ICE1 regulates the cold-induced transcription of CBF/DREB1 genes.
Recognizes the p20 subunit of reduced human caspase 1 isoforms and the p45 proenzyme.
Physical form
Solution of antiserum in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M sodium chloride, and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Kenji Miura et al.
The Plant cell, 19(4), 1403-1414 (2007-04-10)
SIZ1 is a SUMO E3 ligase that facilitates conjugation of SUMO to protein substrates. siz1-2 and siz1-3 T-DNA insertion alleles that caused freezing and chilling sensitivities were complemented genetically by expressing SIZ1, indicating that the SIZ1 is a controller of
Stefano Toldo et al.
Experimental physiology, 98(3), 734-745 (2012-11-28)
The formation of the cryopyrin inflammasome in the heart induces an intense inflammatory response during acute myocardial infarction (AMI), which mediates further damage and promotes adverse cardiac remodelling. Active interleukin-1β (IL-1β) is a key product of the inflammasome, being cleaved
Wang Li Li et al.
Archives of medical science : AMS, 12(5), 950-958 (2016-10-04)
Pattern recognition receptor-mediated signaling pathways have recently been elucidated to bridge the innate immune system and atherosclerosis. NLRP3 is a member of the NLR family. Upon activation, it initiates IL-1β and IL-18 processing, a key step in the inflammatory process
Lili Wang et al.
Archives of medical science : AMS, 10(4), 791-800 (2014-10-03)
Pattern recognition receptor (PRR)-mediated signaling pathways have recently been elucidated to bridge the innate immune system and atherosclerosis. NLRP3 is among the family members of NOD-like receptors (NLRs), a type of PRRs. At present, most studies about the NLRP3 inflammasome
Hwain Shin et al.
Cellular microbiology, 9(12), 2893-2902 (2007-11-10)
Bacteria from the genus Yersinia deliver a number of effectors into host cells via type III secretion (T3S). Injected Yop effectors interfere and prevent pro-inflammatory warning signals by hijacking the host's intracellular machinery. While macrophages infected by wild-type Yersinia enterocolitica
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