Anti-CASK/LIN2 (KA-17) antibody produced in rabbit

CASK/Lin-2 (calcium/calmodulin-dependent serine kinase) has an N-terminal domain similar to calcium-calmodulin dependent protein kinase (CAM), a Lin-7 binding domain, a single PDZ domain, an SH3 domain, and a C-terminal GUK (guanylate kinase) homologous domain. CASK/Lin-2 gene is mapped to human chromosome Xp11.4.

Calcium/Calmodulin-dependent Serine Kinase (CASK/Lin-2) is predominant in the brain, may be ubiquitously expressed. It localizes to the lateral and/or basal plasma membrane regions in epithelial cells.

synthetic peptide corresponding to human CASK/LIN-2 sequence, (amino acids 393-409) conjugated to KLH.

Anti-CASK/LIN2 (KA-17) antibody produced in rabbit is suitable for:

• immunoprecipitation at a concentraqtion of 5-10μg, using lysate of 3x105 cultured MDCK cells
• western blot (chemiluminescent) at a dilution of 1:300, using whole cell extract of cultured human Jurkat and dog MDCK kidney cells

Anti-CASK/Lin-2 specifically recognizes humanCASK/Lin-2.

CASK/Lin-2 (Calcium/Calmodulin-dependent Serine Kinase) is translocated into the nucleus to regulate gene expression. It interacts via its PDZ domain with the cytosolic tail of the transmembrane cell surface heparin sulfate proteoglycan Syndecan-2. In neurons it interacts with neurexins. In addition, CASK/Lin-2 is capable of interacting with the actinbinding band 1 protein, neuronal munc18-1-interacting protein 1(Mint-1/Lin-10), protein lin-7 homolog A (Veli/Lin-7) proteins, junctional adhesion molecule (JAM), disks large homolog 1 (hDlg), and the nuclear T-box brain transcription factor 1 (Tbr-1).

Mutations in the CASK gene is implicated in the X-linked brain malformation syndrome. A missense mutation in the CASK gene is also reported in the pathophysiology of FG syndrome.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

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