生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 34 kDa
种属反应性
mouse, human
技术
immunocytochemistry: suitable
immunohistochemistry (frozen sections): 5 μg/mL
microarray: suitable
western blot: 1 μg/mL using mouse 3T3/A31 and human A431 cell lysates
运输
dry ice
储存温度
−20°C
基因信息
human ... CDC2(983)
mouse ... Cdc2a(12534)
免疫原
synthetic peptide corresponding to the C-terminus of human Cdk1.
应用
Anti-Cdk1 (p34cdc2) antibody produced in rabbit is suitable for:
- detection of CUG-BP1 by immunoblotting and immunocytochemistry.
- western blot at a concentration of 0.25μg/mL using extract of C-2 (mouse myoblasts) cells
- microarray
生化/生理作用
CUG-BP1 protein (hNab50) is part of the large family of nuclear ribonucleoproteins (hnRNPs). It is purified by binding to CUG repeats in the untranslated region of the DMPK mRNA. CUG-BP1 is localized in the cytoplasm and nucleus with different activities. In nuclei, it is involved in splicing of cardiac troponin T (cTnT), insulin receptor, and chloride channel gene transcripts, and its activity is altered in DM1 patients. It regulates translation of a dominant negative isoform of C/EBPβ in the cytoplasm.
The antibody reacts specifically with cyclin-dependent kinase 1 (Cdk1, also termed p34cdc2 and Cdc2). This antibody is not recommended for immunoprecipitation.
外形
Solution in phosphate buffered saline containing 0.05% sodium azide.
法规信息
新产品
此项目有
Johanna R Abend et al.
Virology, 397(1), 73-79 (2009-12-01)
BK virus (BKV) is a ubiquitous human pathogen that establishes a lifelong persistent infection in kidney epithelial cells. BKV reactivation within these cells results in a lytic infection in immunocompromised patients. Little is known about the specific interactions of BKV
Nicolas Charlet-B et al.
Molecular cell, 10(1), 45-53 (2002-08-02)
Myotonic dystrophy type 1 (DM1) is a dominant multisystemic disorder caused by a CTG expansion in the 3' untranslated region of the DMPK gene. A predominant characteristic of DM1 is myotonia resulting from skeletal muscle membrane hyperexcitability. Here we demonstrate
N A Timchenko et al.
The Journal of biological chemistry, 276(11), 7820-7826 (2000-12-23)
An RNA CUG triplet repeat binding protein, CUGBP1, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the 3'-untranslated repeat of the mutant myotonin protein kinase (DMPK) mRNA in myotonic dystrophy (DM) is associated with alterations
Nozomi Sugimoto et al.
The Journal of biological chemistry, 279(19), 19691-19697 (2004-03-03)
The current concept regarding cell cycle regulation of DNA replication is that Cdt1, together with origin recognition complex and CDC6 proteins, constitutes the machinery that loads the minichromosome maintenance complex, a candidate replicative helicase, onto chromatin during the G(1) phase.
L T Timchenko et al.
Nucleic acids research, 24(22), 4407-4414 (1996-11-15)
Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. This study reports the isolation and characterization of a (CUG)n triplet repeat
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