C4977
Caspase 6 human
≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, >1,000 units/mg protein, C-terminal histidine tagged
别名:
Mch2
重组
expressed in E. coli
方案
≥90% (SDS-PAGE)
表单
buffered aqueous solution
比活
>1,000 units/mg protein
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... CASP6(839)
生化/生理作用
Caspases are cysteine aspartic proteases that play a central role in apoptosis. Caspase 6 (Mch2) belongs to the group of effector caspases and is localized downstream of caspase 3. It has been suggested that caspase 6 is implicated in Alzheimer′s disease.
Caspase 6 proenzyme is expressed in E. coli as a C-terminal histidine-tagged protein. It undergoes autoactivation and autoprocessing to 21 and 19 kDa proteins (large subunits), and 14 kDa protein (small subunit). Substrate specificity studies show a favorable interaction of caspase 6 with peptides containing Val in P46. Thus, Ac-VEID-pNA is used as chromogenic substrate for caspase 6.
Caspase 6 proenzyme is expressed in E. coli as a C-terminal histidine-tagged protein. It undergoes autoactivation and autoprocessing to 21 and 19 kDa proteins (large subunits), and 14 kDa protein (small subunit). Substrate specificity studies show a favorable interaction of caspase 6 with peptides containing Val in P46. Thus, Ac-VEID-pNA is used as chromogenic substrate for caspase 6.
外形
Solution in 10% sucrose containing 50 mM HEPES, pH 7.5, 0.5 M NaCl, 100 mM imidazole, 0.1% CHAPS, 4 mM DTT
其他说明
One unit will cleave 1 nmole of Ac-VEID-pNA per minute at 25 °C at pH 7.4.
警示用语：
Danger
危险声明
危险分类
Repr. 1B
储存分类代码
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
E S Alnemri
Journal of cellular biochemistry, 64(1), 33-42 (1997-01-01)
So far nine human aspartate-specific cysteine proteases (ASCPs) have been identified and cloned in our lab and others. Their sequence and structural homology to the nematode Ced-3 implicated them in the cell death pathway of mammalian cells. Recent evidence suggests
H R Stennicke et al.
The Journal of biological chemistry, 272(41), 25719-25723 (1997-11-05)
The observation that the nematode cell death effector gene product Ced-3 is homologous to human interleukin-1beta-converting enzyme (caspase-1) has led to the discovery of at least nine other human caspases, many of which are implicated as mediators of apoptosis. Significant
T Fernandes-Alnemri et al.
Cancer research, 55(13), 2737-2742 (1995-07-01)
We have developed a PCR approach to clone new apoptotic Ced-3/Ice-like cysteine protease genes. This approach uses degenerate oligonucleotides encoding the highly conserved pentapeptides QACRG and GSWFI that are present in all known apoptotic cysteine proteases. Using this approach, we
A LeBlanc et al.
The Journal of biological chemistry, 274(33), 23426-23436 (1999-08-07)
Neuronal cell death, neurofibrillary tangles, and amyloid beta peptide (Abeta) deposition depict Alzheimer's disease (AD) pathology, but neuronal loss correlates best with dementia. We have shown that increased production of Abeta is a consequence of neuronal apoptosis, suggesting that apoptosis
Protein complexes activate distinct caspase cascades in death receptor and stress-induced apoptosis.
S B Bratton et al.
Experimental cell research, 256(1), 27-33 (2000-03-31)
Caspases play a central role in the execution phase of apoptosis and are responsible for many of the morphological features normally associated with this form of cell death. Caspases can activate one another and consequently can initiate specific caspase cascades.
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