Anti-Calpain-94 (Domain II, Insert 1), Large Subunit antibody produced in rabbit

synthetic peptide corresponding to insert 1 in domain-II of the large subunit of human calpain-94 (calpain-3, nCANP, nCL-1, calpain p94, muscle calpain).

Anti-Calpain-94 (Domain II, Insert 1), Large Subunit antibody produced in rabbit is suitable for the following applications:

• Immunohistochemistry (frozen sections)
• Immunoprecipitation
• Indirect ELISA
• Western blot (chemiluminescent) at a dilution of 1:5,000
• Western blotting at a dilution of 1:1,000

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Calpain is a heterodimer consisting of a larger and a smaller subunit. It is a major intracellular protease. Calpains are a family of calcium-dependent thiol-proteases, which are involved in many physiological processes and pathological conditions. These are calcium-activated non-lysosomal thiolproteases. m- and μ-calpain are ubiquitously expressed and are countered by the endogenous calpain inhibitor calpastatin. Calpains are present in all mammalian tissues and are involved in a variety of processes including cell proliferation, differentiation, vesicle secretion and others. It is also likely to be involved in processing of numerous enzymes and cytoskeletal components by linking their activity to a variety of intracellular events.

Recognizes latent and active calpain-94. Binds the reduced and non-reduced protein. Identifies bands at 94 kDa, 82 kDa, 62 kDa, 60 kDa, and a series of further cleaved active forms. The rat 88 kDa and 90 kDa calpains also contain insert 1, so the antibody may cross-react with these forms.

Solution in 0.01 M phosphate buffered saline containing 50% glycerol and 0.05% sodium azide.

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