Monoclonal Anti-Cytokeratin Peptide 17 antibody produced in mouse

Cytokeratin 17, with a molecular weight of 46 kDa, is a member of the type I acidic subfamily of cytokeratins that is expressed in normal human epithelial tissues such as glandular epithelium with myoepithelial component, transitional and pseudostratified epithelia. This protein may be involved in the generation of spatial organization of epithelial tissues.

cytoskeletal preparation from rat colon epithelium.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal Anti-Cytokeratin Peptide 17 antibody produced in mouse is suitable for western blotting, microarray and indirect immunofluorescence at a working dilution of 1:400 using acetone-fixed frozen sections of human salivary gland. It recognizes an epitope present in epithelial tissues and may be used for typing of normal, metaplastic and neoplastic cells. It may be useful in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It may also be used for the localization of cytokeratin 17 using various immunochemical assays such as dot blotting and immunohistochemistry. It has been used in the detection of expression of inducible keratin k17 in human keratinocytes in pachyonychia congenita patients.

Recognizes specifically human cytokeratin 17 (46-47 kDa) and rat cytokeratin 19 (40 kDa). The antibody labels intermediate filaments in human cultured epitheloid carcinoma cell line (HeLa) and in rat hepatoma cells, but does not stain rat fibroblasts in primary cultures. It stains a minor basal cell population in all variants of complex epithelia; strong staining was noted in myoepithelial cells of all glands studied. The antibody exhibits a wide interspecies cross-reactivity, however, the recognized cytokeratin was not studied in cases other than human and rat. The epitope detected by the antibody is sensitive to formalin fixation.

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