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Merck
CN

C9322

过氧化氢酶 来源于牛肝脏

lyophilized powder, 2,000-5,000 units/mg protein

别名:

H2O2:H2O2 氧化还原酶

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化学文摘社编号:
9001-05-2
eCl@ss:
32160410
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-577-1
MDL number:
MFCD00081483
EC 号:
1.11.1.6 (BRENDA, IUBMB)

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产品名称

过氧化氢酶 来源于牛肝脏, lyophilized powder, 2,000-5,000 units/mg protein

SMILES string

O(CC)C(=O)c1ccc(cc1)O

InChI key

NUVBSKCKDOMJSU-UHFFFAOYSA-N

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

biological source

bovine liver

form

lyophilized powder

specific activity

2,000-5,000 units/mg protein

mol wt

tetramer ~250 kDa

isoelectric point

5.4

UniProt accession no.

P00432

shipped in

wet ice

storage temp.

−20°C

Quality Level

400

Gene Information

cow ... CAT(280743)

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Biochem/physiol Actions

本品不需要任何活化剂，但其活性可被3-氨基-1-H-1,2,4三唑、氰化物、叠氮化物、羟胺、溴化氰、2-巯基乙醇、二硫苏糖醇、邻联茴香胺和硝酸盐抑制。
过氧化氢酶是一种存在于所有需氧生物中的抗氧化酶，可将代谢过程的副产物过氧化氢催化降解为危害较小的水和氧气。它还能与烷基过氧化氢反应，如甲基过氧化物和乙基过氧化物，可以用甲醇、乙醇、丙醇、甲酸盐和硝酸盐作为氢供体取代第二 H2O2 分子。过氧化氢酶使用铁（Fe）或锰（Mn）作为辅因子，分为Fe-CAT或Mn-CAT。

Application

过氧化氢酶作为天然抗氧化剂，可用于研究活性氧物质对基因表达和凋亡的作用。它还被用于保护蛋白质、脂质和核酸免受氧化损伤。工业上，过氧化氢酶已被用于去除添加至牛奶和奶酪中以及纺织品漂白使用的过氧化氢，并用于研究其对大肠杆菌DNA修复突变体生活力的正向效应。

牛肝过氧化氢酶可用于：

  • 制备用于葡萄糖生物燃料电池的H2O2-O2基生物阴极
  • 研究固定在硅酸镁载体上的过氧化氢酶的动力学性质和储存稳定性
  • 水溶液中谷胱甘肽介导的超氧化物生成

Disclaimer

过氧化氢酶溶液不可冷冻。 冷冻溶液将导致酶活性损失50-70％。

General description

研究领域：细胞信号传导

牛肝过氧化氢酶是由4个相同亚基组成的四聚体，每个亚基的分子量为60 kDa。每个亚基都含有与原血红素IX基团结合的铁。过氧化氢酶也能紧密结合NADP，其中NADP和血红素基团的距离在13.7埃之内。

Other Notes

一单位酶可在pH7.0、25℃下每分钟分解1.0 μM的H2O2，通过测量A240的下降速率可知H2O2浓度从10.3降至9.2 mM。

Preparation Note

该产品是一种冻干粉，活性是2,000-5,000单位/mg。
该酶溶于50 mM磷酸钾缓冲液中，浓度为1 mg/mL，pH 7.0。
过氧化氢酶溶液不可冷冻。冷冻的过氧化氢酶溶液会损失50-70%活性。建议在-20 °C储存本品的粉末形式。

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品
低风险生物材料
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000517291
0000517291
0000361478
0000376653
0000508024

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can Catalase from bovine liver, Product C9322, be reconstituted?

    This product is soluble in 50 mM potassium phosphate, pH 7.0 at a concentration of 10 mg/mL.

  4. How can a stock solution of Catalase from bovine liver, Product C9322, be stored?

    A 10 mg/mL stock solution prepared in 50 mM potassium phosphate, pH 7.0 and 50% glycerol was stable for at least 1 week in the freezer at -20°C.

  5. What salts are present in Catalase from bovine liver, Product C9322?

    Catalase (C9322) contains potassium phosphate buffered salts (and 20% trehalose as a stabilizer).

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Christopher Gell et al.
Methods in cell biology, 95, 221-245 (2010-05-15)
In vitro assays that reconstitute the dynamic behavior of microtubules provide insight into the roles of microtubule-associated proteins (MAPs) in regulating the growth, shrinkage, and catastrophe of microtubules. The use of total internal reflection fluorescence microscopy with fluorescently labeled tubulin
James C Dooley et al.
Current biology : CB, 30(12), 2404-2410 (2020-05-16)
Cortical development is an activity-dependent process [1-3]. Regarding the role of activity in the developing somatosensory cortex, one persistent debate concerns the importance of sensory feedback from self-generated movements. Specifically, recent studies claim that cortical activity is generated intrinsically, independent
Zhichao Fan et al.
STAR protocols, 1(1), 100012-100012 (2020-10-29)
This protocol introduces the SuperSTORM technique, combining stochastic optical reconstruction microscopy (STORM) and molecular modeling. SuperSTORM is optimized for acquiring and processing STORM images of neutrophil integrins but can be used for any cell-surface molecule with known structure and antibody-binding
Julie Elizabeth Keeble et al.
Pain, 141(1-2), 135-142 (2008-12-09)
Inflammatory diseases associated with pain are often difficult to treat in the clinic due to insufficient understanding of the nociceptive pathways involved. Recently, there has been considerable interest in the role of reactive oxygen species (ROS) in inflammatory disease, but
Amol Aher et al.
Developmental cell, 46(1), 40-58 (2018-06-26)
The dynamic instability of microtubules plays a key role in controlling their organization and function, but the cellular mechanisms regulating this process are poorly understood. Here, we show that cytoplasmic linker-associated proteins (CLASPs) suppress transitions from microtubule growth to shortening
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商品

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

线粒体应激和ROS

氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。

实验方案

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

过氧化氢酶的酶学测定（EC 1.11.1.6）

过氧化氢酶催化过氧化氢生成水和氧气。该实验方案使用分光光度法测定过氧化氢酶活性。

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