产品名称
抗-Cullin-4B 兔抗, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~100 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
western blot: 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CUL4B(8450)
mouse ... Cul4b(72584)
rat ... Cul4b(302502)
相关类别
Application
兔抗-Cullin-4B抗体适用于以表达人Cullin-4B的HEK-293T细胞在0.7-1.5 μg/mL的工作浓度下进行蛋白质印迹实验。在一项证明了CUL4B E3泛素连接酶在靶向Jab1（Jun激活结构域结合蛋白）进行降解并在调节骨形态发生蛋白信号转导中起关键作用的研究中，该抗体被用于免疫印迹。
Biochem/physiol Actions
Cullin-4 （Cul4）蛋白是调节细胞周期、基因组稳定性和DNA复制的泛素E3连接酶的核心成分。Cul4B可作为E3连接酶参与选择泛素化的特异性靶标。它与损伤特异性DNA结合蛋白 DDB1 和DDB2（紫外损伤DNA）有关，并可作为Cul4B E3 连接酶的底物募集因子（substrate-recruiting factor）。Cul4B 可使多种目标蛋白泛素化，包括β-连环蛋白、细胞周期蛋白E、组蛋白H2A。该基因的突变与X连锁智力低下有关。
Cullin-4B可使DNA损伤结合蛋白1 (DDB1) 无需依赖DDB2即可转移进入细胞核。与cullin-4A (Cul4A) 不同，cullin-4B (Cul4B) 不能泛素化DDB2。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
Cullin-4B可形成一种能够充当E3泛素化连接酶的复合体，并催化细胞中特异性蛋白底物的多泛素化。该蛋白可与环指蛋白发生互作，并对多种调节因子的蛋白裂解是必需的
哺乳动物细胞表达两种不同但高度同源的cullin-4蛋白：cullin-4A和cullin-4B。Cul4A和Cul4B组成E3连接酶。与Cul4A相反，Cul4B位于细胞核。
Physical form
溶于含 15 mM 叠氮化钠的 0.01 M 磷酸盐缓冲盐水 (pH 7.4) 中。
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
The Xeroderma Pigmentosum Group E Gene Product DDB2 Is a Specific Target of Cullin 4A in Mammalian Cells
Nag A, et al.
Molecular and Cellular Biology, 21(20), 6738-6747 (2001)
Pengyu Li et al.
Oncology letters, 14(2), 1265-1274 (2017-08-16)
Cullin 4B (Cul4B), a scaffold protein that assembles the ubiquitin ligase complex, is involved in a wide variety of physiological and developmental processes, such as cell cycle progression, DNA damage response and gene expression regulation. Cul4B is overexpressed in various
Rachana Tripathi et al.
Journal of biosciences, 32(6), 1133-1138 (2007-10-24)
Beta-catenin is the key transducer of Wingless-type MMTV integration site family member (Wnt) signalling, upregulation of which is the cause of cancer of the colon and other tissues. In the absence of Wnt signals, beta-catenin is targeted to ubiquitin-proteasome-mediated degradation.
Yongxin Zou et al.
American journal of human genetics, 80(3), 561-566 (2007-02-03)
We reevaluated a previously reported family with an X-linked mental retardation syndrome and attempted to identify the underlying genetic defect. Screening of candidate genes in a 10-Mb region on Xq25 implicated CUL4B as the causative gene. CUL4B encodes a scaffold
Hong Lin et al.
Nature communications, 12(1), 2461-2461 (2021-04-30)
COP1 and COP9 signalosome (CSN) are the substrate receptor and deneddylase of CRL4 E3 ligase, respectively. How they functionally interact remains unclear. Here, we uncover COP1-CSN antagonism during glucose-induced insulin secretion. Heterozygous Csn2WT/K70E mice with partially disrupted binding of IP6
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