biological source
human kidney
packaging
tube of 5 μg 87042204-DNA-5UG, pkg of vial of cells 87042204-1VL
growth mode
Adherent
karyotype
2n = 46, diploid
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Transformation and tumourigenicity studies
Biochem/physiol Actions
Derived from a tumour of a 3 month old male Caucasian. Highly transformed and grows in soft agar. Highly undifferentiated. G401 was originally described as a cell line derived from a Wilm?s tumour. Due to a change in the classification of such tumours, the cell line was examined by Garvin et al., 1993 and found to be more appropriately classified as derived from a rhabdoid tumour of the kidney.
Human Caucasian rhabdoid tumour (formerly classified as Wilm′s tumour)
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 9,14
D16S539: 12
D5S818: 13
D7S820: 11,14
THO1: 8,9.3
TPOX: 8,11
vWA: 16
CSF1PO: 11,13
D13S317: 9,14
D16S539: 12
D5S818: 13
D7S820: 11,14
THO1: 8,9.3
TPOX: 8,11
vWA: 16
Preparation Note
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Change medium every 4 days.
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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