G-401

Transformation and tumourigenicity studies

Derived from a tumour of a 3 month old male Caucasian. Highly transformed and grows in soft agar. Highly undifferentiated. G401 was originally described as a cell line derived from a Wilm?s tumour. Due to a change in the classification of such tumours, the cell line was examined by Garvin et al., 1993 and found to be more appropriately classified as derived from a rhabdoid tumour of the kidney.

Human Caucasian rhabdoid tumour (formerly classified as Wilm′s tumour)

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 9,14
D16S539: 12
D5S818: 13
D7S820: 11,14
THO1: 8,9.3
TPOX: 8,11
vWA: 16

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Change medium every 4 days.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.