AGS Cell Line human

AGS human cell line has been used:

• to study the role of obestatin/G protein-coupled receptor 39 (GPR39) in gastric cancer progression
• to determine expression levels of colon cancer associated transcript -1 (CCAT1)
• to study the adhesion of Lactobacillus sps. to the gastric mucosa

Anti-tumour testing

Derived from an adenocarcinoma of the stomach of a 54 year-old Caucasian female with no prior anti-cancer treatment. AGS cells are persistently infected with parainfluenza virus type 5 (PIV5).

Human Caucasian gastric adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 11,13
D5S818: 9,12
D7S820: 10,11
THO1: 6,7
TPOX: 11,12
vWA: 16,17

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 0.05% EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.