Nthy-ori 3-1 Cell Line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90011609, human thyroid (follicular epithelial), Epithelial
别名:
HTori-3.1 Cells, Nthy-ori 3.1 Cells
生物来源
human thyroid (follicular epithelial)
包装
tube of 5 μg 90011609-DNA-5UG
pkg of vial of cells 90011609-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Nthy-ori 3-1 human cell Line has been used for transfection and virus studies. It is also used for the study of control and growth in human thyroid. The cell line has been used for microRNA (miRNA) expression profiling.
生化/生理作用
Human thyroid follicular epithelial
Normal human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SV-ori) to immortalise them. SV40 DNA was able to immortalise normal thyroid epithelial cells with the retention of several different functions.
制备说明
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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