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NIH 3T3 Cell Line murine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93061524, mouse embryo, Fibroblast

别名:

3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells

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UNSPSC代码:
41106514

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生物来源

mouse embryo

描述

Embryonic fibroblast

包装

tube of 5 μg 93061524-DNA-5UG
pkg of vial of cells 93061524-1VL

生长模式

Adherent

核型

Not specified

形态学

Fibroblast

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

相关类别

应用

DNA transfection studies
NIH 3T3 Cell Line has been used to:
  • study calcium-mediated actin reset (CaAR) in response to physiological changes
  • study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
  • determine the viral titer of the retroviral construct -ST40hTNFα

生化/生理作用

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
Mouse Swiss NIH embryo

制备说明

DMEM + 2mM Glutamine + 10% Calf Serum (CS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

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商品

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DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

实验方案

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