NIH 3T3 Cell Line murine
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93061524, mouse embryo, Fibroblast
别名:
3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells
生物来源
mouse embryo
描述
Embryonic fibroblast
包装
tube of 5 μg 93061524-DNA-5UG
pkg of vial of cells 93061524-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
应用
DNA transfection studies
NIH 3T3 Cell Line has been used to:
- study calcium-mediated actin reset (CaAR) in response to physiological changes
- study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
- determine the viral titer of the retroviral construct -ST40hTNFα
生化/生理作用
Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
Mouse Swiss NIH embryo
制备说明
DMEM + 2mM Glutamine + 10% Calf Serum (CS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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