biological source
mouse tail
packaging
tube of 5 μg 94101211-DNA-5UG, pkg of vial of cells 94101211-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Ecotropic Murine leukemia virus (MuLV)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Detection of replication-competent retroviruses in gene therapy.
Biochem/physiol Actions
Derived from the tail tissue of a normal female mouse, Mus dunni. The cells were cloned at passage 14 using Tererasiki plates. M. dunni (Clone III8C) supports the replication of all four classes of murine leukaemia viruses (MuLV); ecotropic, amphotropic, xenotropic and mink cell focus-forming viruses (MCF), showing cytopathic effect. The cells lack most endogenous murine leukaemia virus-related sequences. They are resistant to infection by Moloney MuLV, due to post-translational modifications of the ecotropic MuLV receptor. Confluent cultures will remain more firmly attached in RPMI 1640 or DMEM + 10% FBS during virus assays.
Mouse tail, normal fibroblast
Preparation Note
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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