STE-137

Isolation of viruses from clinical samples, virus propagation

Fish steelhead trout embryo

STE-137 was initiated in 1963 from pooled steelhead embryos (Oncorynchus mykiss). This heteroploid cell line, also known as steelhead embryo-137, replicates and detects common salmonid viruses (infectious pancreatic necrotic virus (IPNV), infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV)). The growth temperature ranges from 15-21°C with a routine propagation at 18°C.

MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 18°C. Population doubling can take up to 72 hours. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr J L Fryer, Department of Microbiology, Oregon State University, Corvallis

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.