产品名称
脱氧核糖核酸 钠盐 来源于小牛胸腺, Type I, fibers
type
Type I
form
fibers
color
white
storage temp.
2-8°C
Quality Level
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Preparation Note
该产物采用可引起剪切的方法进行提取，产生双链和单链DNA的高度聚合混合物。 但是，双链DNA是主要形式。
DNA溶液已在4℃，pH 7.5-8，含1mM EDTA且不含抑菌剂的10mM Tris中成功保存数月。 在低浓度（μg/ml）条件下，DNA倾向于吸附在塑料管表面上。不建议将DNA储存在高碱性溶液中，因为DNA在pH大于8.0的碱性条件下会降解。
本品能够以2 mg/ml的浓度溶于水。为了进一步减少DNA剪切，不应使用超声处理或搅拌。 建议在0-4°C轻轻摇晃过夜，以便完全溶解DNA。 建议添加1 mM EDTA，以防止核酸酶降解DNA。
本品采用未指定性别的小牛的胸腺组织制备。
Analysis Note
“高度聚合”
Application
小牛胸腺DNA（CT-DNA）是一种天然DNA，广泛用于研究调节DNA结构和功能的DNA结合抗癌剂和DNA结合剂。 小牛胸腺DNA也用于溶液中DNA行为的物理化学研究。
General description
λmax 259 nm（100 mM磷酸盐缓冲液，pH7.0）。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0，对应于约50 μg的双链DNA。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0，对应于约50 μg的双链DNA。
Physical form
纤维状制剂
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
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实验方案
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
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