D1501
脱氧核糖核酸 钠盐 来源于小牛胸腺
Type I, fibers
别名:
ctDNA, 胸腺核酸 钠盐, 脱氧核糖核酸 钠盐 来源于小牛胸腺
一般描述
λmax 259 nm（100 mM磷酸盐缓冲液，pH7.0）。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0，对应于约50 μg的双链DNA。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0，对应于约50 μg的双链DNA。
应用
小牛胸腺DNA（CT-DNA）是一种天然DNA，广泛用于研究调节DNA结构和功能的DNA结合抗癌剂和DNA结合剂。 小牛胸腺DNA也用于溶液中DNA行为的物理化学研究。
外形
纤维状制剂
制备说明
DNA溶液已在4℃，pH 7.5-8，含1mM EDTA且不含抑菌剂的10mM Tris中成功保存数月。 在低浓度（μg/ml）条件下，DNA倾向于吸附在塑料管表面上。不建议将DNA储存在高碱性溶液中，因为DNA在pH大于8.0的碱性条件下会降解。
本品能够以2 mg/ml的浓度溶于水。为了进一步减少DNA剪切，不应使用超声处理或搅拌。 建议在0-4°C轻轻摇晃过夜，以便完全溶解DNA。 建议添加1 mM EDTA，以防止核酸酶降解DNA。
本品采用未指定性别的小牛的胸腺组织制备。
该产物采用可引起剪切的方法进行提取，产生双链和单链DNA的高度聚合混合物。 但是，双链DNA是主要形式。
分析说明
“高度聚合”
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Oskar Szczepaniak et al.
Biomolecules, 10(5) (2020-05-06)
Since ancient times, fruits and edible plants have played a special role in the human diet for enhancing health and maintaining youthfulness. The aim of our work was to determine the interactions between naringin, a natural ingredient of grapefruits, and
A M Parker et al.
Journal of dairy science, 99(12), 9875-9884 (2016-10-04)
Bacterial contamination of milk fed to calves compromises calf health. Several bacterial pathogens that infect cows, including Mycoplasma bovis and Salmonella enterica ssp. enterica serovar Dublin, are shed in milk, providing a possible route of transmission to calves. Milk acidification
M S Hazelton et al.
Journal of dairy science, 101(8), 7412-7424 (2018-05-14)
With the common use of bulls for breeding following a period of artificial insemination in seasonally bred dairy herds, it is important to consider the potential role of the bull in transmission of Mycoplasma spp. within and between herds. This
M S Hazelton et al.
Journal of dairy science, 103(12), 11844-11856 (2020-09-29)
Replacement dairy heifers exposed to Mycoplasma bovis as calves may be at risk of future clinical disease and pathogen transmission, both within and between herds; however, little information is available about these risks. We conducted a 2-yr longitudinal (panel) study
Hironobu Ikehata et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 17(4), 404-413 (2018-02-22)
The amount of photolesions produced in DNA after exposure to physiological doses of ultraviolet radiation (UVR) can be estimated with high sensitivity and at low cost through an immunological assay, ELISA, which, however, provides only a relative estimate that cannot
实验方案
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
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