跳转至内容
Merck
CN

D1501

脱氧核糖核酸 钠盐 来源于小牛胸腺

Type I, fibers

别名:

ctDNA, 胸腺核酸 钠盐, 脱氧核糖核酸 钠盐 来源于小牛胸腺

登录 查看公司和协议定价

选择尺寸


关于此项目

化学文摘社编号:
MDL编号:
UNSPSC代码:
41106305
eCl@ss:
32160414
NACRES:
NA.51
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

一般描述

λmax 259 nm(100 mM磷酸盐缓冲液,pH7.0)。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0,对应于约50 μg的双链DNA。

应用

小牛胸腺DNA(CT-DNA)是一种天然DNA,广泛用于研究调节DNA结构和功能的DNA结合抗癌剂和DNA结合剂。 小牛胸腺DNA也用于溶液中DNA行为的物理化学研究。

外形

纤维状制剂

制备说明

DNA溶液已在4℃,pH 7.5-8,含1mM EDTA且不含抑菌剂的10mM Tris中成功保存数月。 在低浓度(μg/ml)条件下,DNA倾向于吸附在塑料管表面上。不建议将DNA储存在高碱性溶液中,因为DNA在pH大于8.0的碱性条件下会降解。
本品能够以2 mg/ml的浓度溶于水。为了进一步减少DNA剪切,不应使用超声处理或搅拌。 建议在0-4°C轻轻摇晃过夜,以便完全溶解DNA。 建议添加1 mM EDTA,以防止核酸酶降解DNA。
本品采用未指定性别的小牛的胸腺组织制备。
该产物采用可引起剪切的方法进行提取,产生双链和单链DNA的高度聚合混合物。 但是,双链DNA是主要形式。

分析说明

“高度聚合”

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Valeria Visone et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 1363-1371 (2020-06-20)
Cancer is a major health issue adsorbing the attention of a biomedical research. To fight this disease, new drugs are developed, specifically tailored to target biological pathways or peculiar components of the tumour cells. Particularly interesting is the use of
Alysia M Parker et al.
Veterinary microbiology, 196, 118-125 (2016-12-13)
Mycoplasma bovis is a major pathogen in cattle causing mastitis, arthritis and pneumonia. First isolated in Australian cattle in 1970, M. bovis has persisted causing serious disease in infected herds. To date, genetic analysis of Australian M. bovis isolates has
Hironobu Ikehata et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 17(4), 404-413 (2018-02-22)
The amount of photolesions produced in DNA after exposure to physiological doses of ultraviolet radiation (UVR) can be estimated with high sensitivity and at low cost through an immunological assay, ELISA, which, however, provides only a relative estimate that cannot
M S Hazelton et al.
Journal of dairy science, 101(8), 7412-7424 (2018-05-14)
With the common use of bulls for breeding following a period of artificial insemination in seasonally bred dairy herds, it is important to consider the potential role of the bull in transmission of Mycoplasma spp. within and between herds. This
A M Parker et al.
Journal of dairy science, 99(12), 9875-9884 (2016-10-04)
Bacterial contamination of milk fed to calves compromises calf health. Several bacterial pathogens that infect cows, including Mycoplasma bovis and Salmonella enterica ssp. enterica serovar Dublin, are shed in milk, providing a possible route of transmission to calves. Milk acidification

实验方案

Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持