D4822
杜氏改良 Eagle 培养基 - 高葡萄糖
HEPES modification, With 4500 mg/L glucose, L-glutamine, 25 mM HEPES, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
别名:
DME, DMEM
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
cell culture | stem cell: suitable
杂质
Endotoxin, tested
组分
phenol red: yes
L-glutamine: yes
sodium pyruvate: no
glucose: high
运输
ambient
储存温度
2-8°C
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应用
Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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