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Molecular Biology
form
buffered aqueous solution
mol wt
5500 bp
storage temp.
−20°C
General description
pcDNA3.1 (-)/myc-his C is an expression vector that contains the strong CMV enhancer-promoter for high level expression of recombinant proteins in mammalian cells. The pcDNA3.1(-)/myc-his C expression vector contains a multiple cloning site (MCS) for easy cloning. pcDNA3.1(-) /myc-his C expression vector has a multiple cloning site in an opposite orientation from the pcDNA3.1(+). The vector is supplied in one of three reading frames to facilitate in frame cloning with a C-terminal tag containing a polyhistidine metal-binding region and the myc (c-myc) epitope.
The MCS has the following unique restriction sites: Apa I, Asp718 I, BamH I, BstX I, EcoR I, EcoR V, Hind III, KpN I, Nhe I, Not I, Pme I, Xba I, and Xho I. In addition, the SnaBI site in the CMV promoter is unique and the BstXI site in the MCS is redundant.
The MCS has the following unique restriction sites: Apa I, Asp718 I, BamH I, BstX I, EcoR I, EcoR V, Hind III, KpN I, Nhe I, Not I, Pme I, Xba I, and Xho I. In addition, the SnaBI site in the CMV promoter is unique and the BstXI site in the MCS is redundant.
Application
pcDNA 3.1 (-)/Mys-His C Expression Vector is suitable for expression of recombinant proteins in mammalian cells.
Biochem/physiol Actions
The MCS, containing Apa I, Asp718 I, BamH I, BstX I, EcoR I, EcoR V, Hind III, KpN I, Nhe I, Not I, Pme I, Xba I, Xho I restriction sites, is in the reverse orientation. In addition, there is a unique SnaB I site in the CMV promoter.
Features and Benefits
- CMV promoter/enhancer permits efficient, high-level expression
- MCS facilitates easy insertion of your gene
- Myc epitope allows recombinant protein detection
- His-tag allows recombinant protein purification (on metal chelate resins)
- Confers ampicillin resistance for selection of transformed bacteria
- Confers neomycin resistance for selection of tranfected mammalian cells
- SV40 promoter allows high level expression of inserted gene and episomal replication in cells expressing the large T antigen
A 5.5-kb vector for protein expression in mammalian cells. The vector incorporates both the myc epitope and polyHistidine in a C-terminal fusion protein tag. The A, B, and C versions differ in only a single base pair, allowing for translation in all possible reading frames.
All three contain the strong cytomegalovirus (CMV) enhancer-promoter sequence for high-level expression, the polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability, and the SV40 replication origin.
All three contain the strong cytomegalovirus (CMV) enhancer-promoter sequence for high-level expression, the polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability, and the SV40 replication origin.
Other Notes
pcDNA 3.1 (-)/Mys-His C Expression Vector is supplied lyophilized in 10 mM Tris-HCl and 1 mM EDTA (pH 7.5).
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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