recombinant
expressed in Sf9 cells
packaging
vial of >25 units
concentration
≥0.33 unit/μL (band-forming units, BFU)
UniProt accession no.
storage temp.
−70°C
Gene Information
human ... EGR3(1960)
Application
Early Growth Response proteins expressed in insect cells show superior performance in Electrophoretic Mobility Shift Assay (EMSA) compared to proteins expressed in E. coli. May be used for in vitro transcription assays and analysis of nuclear extracts.
Biochem/physiol Actions
EGR (Early Growth Response) proteins represent a family of transcription factors involved in cell cycle regulation. They contain three, nearly identical DNA-binding zinc finger regions, each possessing a unique flanking region. EGR-1, -2, -3, and -4, bind the EGR-consensus sequence GCG T/GGG GCG, however the individual proteins show differing binding affinities.
Physical form
Solution in 20 mM HEPES, 420 mM NaCl, 0.2 mM EDTA, 25% glycerol, and 1.5 mM MgCl2, pH 7.9.
法规信息
新产品
此项目有
S Patwardhan et al.
Oncogene, 6(6), 917-928 (1991-06-01)
We have previously described two cellular immediate-early genes, Egr-1 (mouse) and EGR2 (human) that encode zinc finger proteins. Here we report the characterization of a new member of the Egr family referred to as EGR3 (human). This cDNA clone was
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