Anti-ERGIC-53/p58−Cy3 antibody produced in rabbit

Anti-ERGIC-53/p58-Cy3 antibody produced in rabbit is suitable for direct immunofluorescence at a concentration of 0.5-1.0μg/mL using human HeLa cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Protein ERGIC-53 (also known as ER-Golgi intermediate compartment 53 kDa protein or lectin mannose-binding 1) is a type I integral membrane protein encoded by the LMAN1 gene in humans. ERGIC-53 is a component of ER-Golgi intermediate compartment and is mapped to a YAC and BAC contig containing the critical region for the combined factors V and VIII deficiency gene. It is a dynamic membrane system composed of a number of clusters. ERGIC-53 bypasses the major recycling pathway through Golgi apparatus. It may function as a molecular chaperone during transportation of a specific subset of secreted proteins including coagulation factors V and VIII from ER to Golgi.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Protect from prolonged exposure to light. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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