tag
FLAG® tagged
grade
Molecular Biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
General description
The pFLAG-MAC™ Expression Vector is a 5.0 kb E. coli expression vector used for cloning and cytoplasmic expression of properly inserted open reading frame as an N-terminal Met-FLAG® fusion protein. The Met-FLAG fusion protein may be detected using Monoclonal ANTI-FLAG M2 (F3165), Monoclonal ANTI-FLAG M5 (F4042), and can be purified using the ANTI-FLAG M2 Affinity Gel (A2220).
The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein. The FLAG-BAP fusion protein may be detected using Monoclonal ANTI-FLAG M1 (F3040), or Monoclonal ANTI-FLAG M2 (F3165), or purified using ANTI-FLAG M1 Agarose Affinity Gel (A4596), or ANTI-FLAG M2 Affinity Gel (A2220).
Vector Maps and Sequences
The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein. The FLAG-BAP fusion protein may be detected using Monoclonal ANTI-FLAG M1 (F3040), or Monoclonal ANTI-FLAG M2 (F3165), or purified using ANTI-FLAG M1 Agarose Affinity Gel (A4596), or ANTI-FLAG M2 Affinity Gel (A2220).
Vector Maps and Sequences
Other Notes
- pFLAG-MAC™ Expression Vector 10 μg (E5644) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pFLAG-ATS-BAP Control Plasmid 1 μg (C9079) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-MAC is a trademark of Sigma-Aldrich Co. LLC
存储类别
10 - Combustible liquids
法规信息
新产品
此项目有
Shu-Hua Huang et al.
Gene, 766, 145156-145156 (2020-09-20)
Plant Glycogen Synthase Kinase 3 (GSK3)/SHAGGY-like kinase (GSK) proteins play important roles in modulating growth, development, and stress responses in several plant species. However, little is known about the members of the potato GSK (StGSK) family. Here, nine StGSK genes
H Komatsuzawa et al.
The Journal of antimicrobial chemotherapy, 45(4), 421-431 (2000-07-15)
A Tn551 insert in a gene termed fmtB was shown to reduce oxacillin as well as Triton X-100 resistance in highly methicillin-resistant Staphylococcus aureus (MRSA) COL. Backcrosses of fmtB::Tn551 into S. aureus COL and into two genetically distinct MRSA strains
Sandra Lobo et al.
The Journal of biological chemistry, 277(43), 41268-41273 (2002-08-24)
Most Ras proteins are posttranslationally modified by a palmitoyl lipid moiety through a thioester linkage. However, the mechanism by which this occurs is not known. Here, evidence is presented that the Ras2 protein of Saccharomyces cerevisiae is palmitoylated by a
Julia A Ross et al.
Journal of bacteriology, 193(9), 2276-2289 (2011-03-02)
YscD is an essential component of the plasmid pCD1-encoded type III secretion system (T3SS) of Yersinia pestis. YscD has a single transmembrane (TM) domain that connects a small N-terminal cytoplasmic region (residues 1 to 121) to a larger periplasmic region
Vikramjit S Bajwa et al.
Plant physiology, 163(1), 30-42 (2013-07-12)
Brassinosteroids (BRs) are plant hormones that are perceived at the cell surface by a membrane-bound receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1). BRI1 interacts with BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1) to initiate a signal transduction pathway in which autophosphorylation and transphosphorylation of BRI1
相关内容
pFLAG-MAC and pFLAG-ATS
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持