EphA3/Fc Chimera from mouse

EPHA3 is a receptor tyrosine kinase (RTK) belonging to the largest subgroup of RTKs called ephrin group of receptors. It has a high level of expression from placental stage through the whole developmental process. It is a type I transmembrane protein, and has a membrane-bound ligand. It is both an oncogene and a tumor-suppressor gene. This protein resides in both the cytosol and the plasma membrane.

EPHA3 binds the ephrin-A ligand and has diverse cellular function. Analysis of human colorectal, breast, lung and pancreatic cancer samples shows somatic mutations in the EPHA3 gene. EPHA3 gene expression can be regulated by CD28 and IGF-1 in Jurkat cells and expression of EPHA3 is associated with adherence and motility of malignant T cells. It maintains the less differentiated state of cancer cells, by activating MAPK (mitogen-activated protein kinase) pathway. It is over-expressed in glioblastoma (GBM) cells, especially during the initial stages. It also higher expression levels in aggressive mesenchymal subtype of GBM. In hepatocellular carcinoma (HCC), it predicts increased invasiveness and poor patient prognosis.

Member of the Eph receptor tyrosine kinase family shown to bind ephrin-A1, ephrin-A2, ephrin-A3, ephrin-A4, ephrin-A5, and ephrin-B1; involved in pattern formation and morphogenesis.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

The biological activity is measured by its ability to bind recombinant human ephrin-A5/Fc in an ELISA.

Extracellular domain of mouse EphA3 (amino acids 1-541) fused by means of a polypeptide linker to the Fc region of human IgG1 that is histidine tagged at the C-terminus.