biological source
mouse
recombinant
expressed in NSO cells
assay
>95% (SDS-PAGE)
form
lyophilized powder
mol wt
monomer calculated mol wt 85.2 kDa, ~110 kDa by SDS-PAGE (reducing)
storage condition
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
technique(s)
ligand binding assay: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Ephb4(13846)
General description
EPHB4 (Ephb1 Eph receptor B4) belongs to EPHB receptor family, which is the biggest family of transmembrane tyrosine kinase receptors. Mammals contain five EPHB receptors, namely, EPHB1-EPHB4 and EPHB6. Their ligands are transmembrane in nature and are called ephrinB1, -B2 and -B3.
Biochem/physiol Actions
EPHB4 is a member of the Eph family of receptor tyrosine kinases, which play a role in regulating cell adhesion and cell movement during embryonic development. Furthermore, because tissue disorganization and abnormal cell adhesion, movement, and survival characterize the more advanced stages of cancer, the inappropriate functioning of Eph receptor could play a role in malignancy. Furthermore, EPHB4 has been shown to be the major essential interaction partner of Ephrin B2 in angiogenesis.
Member of Eph receptor tyrosine kinase family shown to bind ephrin-B1 and ephrin-B2; involved in pattern formation and morphogenesis and arterial/venous demarcation of the developing vasculature. EphB4 is also involved in hematopoiesis.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
Analysis Note
The biological activity is measured by its ability to bind recombinant mouse ephrin-B2/Fc in an ELISA assay.
Other Notes
Extracellular domain of mouse EphB4 (amino acids 1-539) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
A C Andres et al.
Oncogene, 9(5), 1461-1467 (1994-05-01)
To investigate the involvement of protein tyrosine kinases (PTKs) in the growth control of mammary epithelial cells, we have used PCR based cloning to identify PTKs expressed in a mouse mammary epithelial cell line. This approach led to the isolation
S R Kumar et al.
British journal of cancer, 96(7), 1083-1091 (2007-03-14)
EphB4 is a member of the largest family of transmembrane receptor tyrosine kinases and plays critical roles in axonal pathfinding and blood vessel maturation. We wanted to determine the biological role of EphB4 in ovarian cancer. We studied the expression
Vincent Cibert-Goton et al.
PloS one, 8(1), e53673-e53673 (2013-01-24)
EphB receptors tyrosine kinases and ephrinB ligands were first identified as guidance molecules involved in the establishment of topographical mapping and connectivity in the nervous system during development. Later in development and into adulthood their primary role would switch from
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持