biological source
mouse
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
purified by
affinity chromatography (affinity tag purification)
species reactivity
all
concentration
1 mg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
wet ice
storage temp.
−20°C
Quality Level
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General description
小鼠抗-FLAG M2抗体,可与含有FLAG肽序列结合的亲和纯化单克隆抗体。该抗体可识别FLAG肽序列的N-末端、Met-N-末端、C-末端和融合蛋白的内部位点。
纯化方法 - 亲和标签纯化
纯化方法 - 亲和标签纯化
Immunogen
FLAG;肽序列DYKDDDDK
Application
用于通过免疫印迹、免疫沉淀(IP)、免疫组化、免疫荧光及免疫细胞化学进行FLAG融合蛋白的高灵敏度及特异性检测。 优化用于哺乳动物、植物和细菌表达系统中FLAG融合蛋白的单条带检测。
蛋白质免疫印迹和EIA
更多产品信息请访问我们的FLAG®应用页面。
蛋白质免疫印迹和EIA
更多产品信息请访问我们的FLAG®应用页面。
Biochem/physiol Actions
结合位点:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Physical form
在磷酸盐缓冲液中含有50%甘油。
Preparation Note
在立即使用前用3%脱脂牛奶稀释于Tris缓冲盐溶液(TBS),pH 8.0
Other Notes
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
10 - Combustible liquids
法规信息
低风险生物材料
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商品
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
免疫印迹(蛋白质印迹转印)是现代蛋白质组学研究中的常用技术。
相关内容
蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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