F3174
Fpg Protein from Escherichia coli
≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis
别名:
DNA-(apurinic or apyrimidinic site)lyase MutM (APlyase MutM), Fapy-DNAglycosylase, Formamidopyrimidine-DNA glycosylase, Fpg Protein from Escherichia coli, Recombinant, Fapy DNA glycosylase, Formamidopyrimidine DNA glycosylase, MutM
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Form:
buffered aqueous glycerol solution
Assay:
≥90% (SDS-PAGE)
Biological source:
Escherichia coli
Recombinant:
expressed in E. coli
Mol wt:
30.2 kDa (269 amino acids, predicted from the nucleotide sequence)
biological source
Escherichia coli
Quality Segment
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
>20,000 units/mg protein
mol wt
30.2 kDa (269 amino acids, predicted from the nucleotide sequence)
composition
protein, 0.1- 0.3 mg/mL Bradford
storage condition
(Tightly closed)
technique(s)
nucleic acid detection: suitable
UniProt accession no.
application(s)
genomic analysis
shipped in
wet ice
storage temp.
−20°C
Gene Information
Escherichia coli CFT073 ... mutM(1038243)
Escherichia coli K12 ... mutM(946765)
General description
Fpg contains two domains separated by a flexible hinge.
Research area: Cell signaling
Research area: Cell signaling
Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.
Application
Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.
Biochem/physiol Actions
Fpg specifically acts on 3′- and 5′-phosphodiester bonds.
Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.
Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.
Physical form
Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.
Other Notes
One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 °C.
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)