Anti-GABA_B Receptor 1 antibody produced in rabbit

Gamma-aminobutyric acid (GABA) receptor is the multifunctional, pentameric membrane proteins that operate GABA-gated chloride channels. It acts as major inhibitory neurotransmitter in the adult mammalian brain. It is a member of the neurotransmitter ligand-gated ion channels which plays various functions in the central nervous system, the peripheral nervous system, and in some non-neuronal tissues. At least four types of subunits (α, β, γ and δ) of GABA have been identified. Generally it contains one type of α and β subunit, and a single γ polypeptide in a ratio of 2:2:1.

synthetic peptide corresponding to the N-terminal extracellular domain of human GABA_B Receptor 1, conjugated to KLH. The immunizing peptide has 100% homology with the rat and mouse gene.

Anti-GABA_B Receptor antibody is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a working concentration of 9-18μg/mL using human cerebellum, Purkinje.

GABA is synthesized primarily from glutamate by glutamate decarboxylase (GAD). Its function is triggered by binding to its ionotropic receptors, GABA(A) and GABA(C), which are ligand-gated chloride channels, and its metabotropic receptor, GABA(B). Along with neural development, it is also involved in a wide variety of physiological functions in tissues and organs outside the brain. GABA_B broadly expressed in the nervous system. It binds and activates the guanine nucleotide-binding proteins as a second messenger. It has been reported that presynaptic GABA_B receptors can inhibit Ca²⁺ channels in a membrane-delimited manner via the Gβγ subunits. On the other hand, postsynaptic GABA_B receptors trigger the opening of K⁺ channels via the Gβγ subunits. GABA_B is also associated with the activation and inhibition of adenylyl cyclase.

Solution in phosphate buffered saline, pH 7.7, containing 0.01% sodium azide.