G4045
Anti-GRP75 (SQ-15) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-Glucose Regulated Protein 75, Anti-Mitochondrial Hsp70, Anti-Mortalin, Anti-Peptide Binding Protein 74
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 75 kDa
种属反应性
mouse, human, canine
浓度
~1 mg/mL
技术
indirect immunofluorescence: 1-2 μg/mL using HeLa cells
western blot: 0.2-0.4 μg/mL using whole cell extracts of HeLa, MDCK, and NIH3T3 cells
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
mouse ... Hspa9(15526)
rat ... Hspa9(291671)
一般描述
GRP75 (SQ-15) encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation.
GRP75 (glucose-regulated protein 75) belongs to a Hsp70 family of the heat shock proteins.
GRPs are unresponsive to heat stress and are induced by stress related to glucose starvation or defects in glycoprotein processing. GRP75/mortalin has been localized mainly to the endoplasmic reticulum (ER), but also to various cellular compartments including mitochondria and cytoplasmic vesicles.
免疫原
synthetic peptide corresponding to amino acids 665-679 located at the C-terminus of human GRP75. This sequence is identical in mouse GRP75 (mot-1 and mot-2 isoforms) and highly conserved in rat (1 amino acid substitution).
应用
Anti-GRP75 antibody produced in rabbit has been used in :
- immunofluorescence
- immunoblotting
- immunocytochemistry
生化/生理作用
GRP75 (glucose-regulated protein 75) is involved in stress responses to intracellular trafficking, antigen processing, and control of cell proliferation, differentiation, and tumorigenesis. It is induced by low levels of ionizing radiation, glucose deprivation, calcium ionophore, and ozone and its levels correlate with muscle activity, mitochondrial activity, and biogenesis.
Heat shock proteins (HSP) are considered to function as molecular chaperones by transiently binding to newly synthesized proteins to facilitate their correct folding and assembly. GRP75 levels are associated with muscle activity, mitochondrial function and biogenesis.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Hsp70 family member, mot-2/mthsp70/GRP75, binds to the cytoplasmic sequestration domain of the p53 protein
Wadhwa R, et al.
Experimental Cell Research, 274(2), 246-253 (2002)
Mortalin is expressed by astrocytes and decreased in the midbrain of Parkinson's disease patients
Cook TJ, et al.
Brain Pathology, 26(1), 75-81 (2016)
Organelle-associated rRNA Degradation
Parekh VS
The Journal of Biological Chemistry (2018)
Molecular chaperones in cellular protein folding
Hartl EU, et al.
Nature, 381(6583), 571-571 (1996)
Jinliang Huang et al.
Bio-protocol, 9(11), e3255-e3255 (2019-06-05)
Cytosolic rRNAs are highly dynamic and can be degraded under conditions such as apoptosis, starvation and magnesium depletion. The degradation is also related to their specific localization, as fractions of cytosolic ribosomes are localized on the surfaces of intracellular organelles
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