Anti-Gα_i1,2 antibody produced in rabbit

G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and γ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands. All α subunits appear to be palmitoylated near the N-terminus. Palmitate regulates GTPase activity by GTPase activating proteins.

Synthetic peptide corresponding to the C-terminal region of Gα _i1,2.

Anti-Gα_i1,2 antibody produced in rabbit is suitable for use as a primary antibody at a working dilution of 1:1000 in immunocytochemical staining of oocytes of the starfish Asterias amurensis after induction of maturation.

The gene GNAI2 (guanine nucleotide binding protein (G protein), α inhibiting activity polypeptide 2) encodes an α subunit of guanine nucleotide binding proteins. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βγ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βγ-subunit complex. This subunit has the binding site for guanine nucleotide and regulates adenylate cyclase. G α_i-2 is localized to the basolateral plasma membrane.Carboxy-terminal end plays a key role in the specificity of membrane targeting in G_{α i} proteins. When coupled to phospholipase C, the α subunits may have a role in pertussis toxin-insensitive pathways.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.