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安全信息

G7765

Sigma-Aldrich

明胶 来源于冷水鱼类的皮肤

40-50% in H2O

别名:

白明胶, 硬骨素明胶

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About This Item

CAS号:
9000-70-8
MDL编号:
MFCD00081638
UNSPSC代码:
12352202
NACRES:
NA.61

生物来源

fish skin

质量水平

200

表单

liquid

分子量

~60 kDa

包含

0.15% propyl p-hydroxybenzoate as preservative
0.2% methyl p-hydroxybenzoate as preservative

浓度

40-50% in H2O

技术

ELISA: suitable
cell culture | mammalian: suitable
immunocytochemistry: suitable
western blot: suitable

储存温度

2-8°C

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一般描述

明胶存在于胶原蛋白中，是一种具有高平均分子量的水溶性蛋白质的异质性混合物。通过在水中将皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。
明胶来源于胶原蛋白，是组织胞外基质(ECM)中含量最丰富的蛋白质。它是一种生物活性物质。明胶由基质金属蛋白酶(MMP)敏感的蛋白序列组成。

应用

来自冷水鱼皮的明胶已用于：大鼠大脑切片免疫标记的孵育培养液(IM)，用作各种细胞蛋白质印迹的洗涤液组分；还用作HeLa细胞免疫荧光染色的阻断剂。
在免疫组化中用作封闭剂。

生化/生理作用

明胶是一种化学或酶促交联形成的三维(3D)水凝胶。

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

明胶类产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000429453
0000429453
0000418907
0000418907
0000425718

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Product G7765, Gelatin from cold water fish skin, soluble in?      

    Gelatin is soluble in glycerol and acetic acid, and more soluble in hot water than in cold water.

  4. What is the solution stability of Product G7765, Gelatin from cold water fish skin?

    The specification for shelf life is 2 years for unopened bottles stored at 2-8°C.  Each lot is assigned a retest date; this date is given on the Certificate of Analysis avialable from our website.

  5. What is the difference between type A and type B gelatin?

    Type A gelatin is derived from acid-cured tissue whereas Type B gelatin is derived from lime-cured tissue. Type A gelatin has about 78-80 millimoles of free carboxyl groups per 100 g of protein and a pI of 7.9-9.0. Type B has 100-115 millimoles of free carboxyl groups per 100 g of protein and a pI of 4.7-5.2. 

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. What is the gel point of Product G7765, Gelatin from cold water fish skin?

    The gel point of Product G7765, Gelatin from cold water fish skin is 5-10°C. The product will be a gel when storing the product at 2-8°C.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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