G7882
L-谷氨酸脱氢酶 来源于牛肝脏
Type III, lyophilized powder, ≥20 units/mg protein
别名:
L -GLDH, L -谷氨酸盐:NAD[P]+ 氧化还原酶(脱氨), 谷氨酸脱氢酶 来源于牛肝脏
生物来源
bovine liver
质量水平
类型
Type III
表单
lyophilized powder
比活
≥20 units/mg protein
UniProt登记号
储存温度
−20°C
基因信息
cow ... GLUD1(281785)
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应用
L-谷氨酸脱氢酶可用于催化异柠檬酸转化为α-酮戊二酸和二氧化碳。
生化/生理作用
这种酶的哺乳动物形式,包括这种牛形式,可以使用 NADP (H) 或 NAD (H) 作为辅酶。 L -谷氨酸脱氢酶在哺乳动物代谢中起着独特角色。该酶催化的逆向反应是氨与 α-淀粉结合的唯一途径。-α 的碳原子-因此,羧酸是哺乳动物中从头合成氨基酸的唯一来源。
牛酶的特点有三组属性:
L-谷氨酸脱氢酶催化谷氨酸转化为 α-酮戊二酸 。
牛酶的特点有三组属性:
- 具有可逆的浓度依赖性结合,产生较高分子量形式。
- 形成紧密的酶-还原型辅酶-底物三元复合物,其解离速率调节稳态反应速率。
- 表现出各种各样的影响,从结合的任何编号核苷酸修饰。
L-谷氨酸脱氢酶催化谷氨酸转化为 α-酮戊二酸 。
包装
包装尺寸基于蛋白质含量
外形
含有柠檬酸盐和磷酸钾缓冲盐。
分析说明
双缩脲法蛋白测定
其他说明
在25℃、pH 7.3、铵离子存在的条件下,一单位酶每分钟可将1.0 μmole α-酮戊二酸还原成 L-谷氨酸。
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Roy M Daniel et al.
The Biochemical journal, 425(2), 353-360 (2009-10-24)
Experimental data show that the effect of temperature on enzymes cannot be adequately explained in terms of a two-state model based on increases in activity and denaturation. The Equilibrium Model provides a quantitative explanation of enzyme thermal behaviour under reaction
Decreased carbohydrate metabolism enzyme activities in the glaucomatous trabecular meshwork
Junk AK, Goel M, Mundorf T, Rockwood EJ, Bhattacharya SK
Molecular Vision, 10, 1286-1291 (2010)
Laszlo Tretter et al.
Journal of neurochemistry, 83(4), 855-862 (2002-11-08)
Previously we have reported that oxidative stress induced by hydrogen peroxide exacerbates the effect of an Na+ load in isolated nerve terminals, with a consequence of an ATP depletion, [Ca2+]i and [Na+]i deregulation, and collapse of mitochondrial membrane potential. In
S M Kuo et al.
Biology of the neonate, 43(1-2), 23-32 (1983-01-01)
The tissue distribution, the subcellular distribution in liver, and the developmental patterns of cysteine:alpha-ketoglutarate aminotransferase (CAT) and 3-mercaptopyruvate sulfurtransferase (MPST) activities were determined in rats of the Sprague-Dawley strain. CAT activity was highest in heart and liver, whereas MPST activity
James K Kranz et al.
Methods in molecular biology (Clifton, N.J.), 796, 3-17 (2011-11-05)
Thermodynamic principles of cooperativity and allostery have long been used as a starting point to begin understanding the interplay between ligand binding events. Understanding the nature of allosteric effects requires an experimental technique that can be used to quantify ligand
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