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Merck
CN

G7882

L-谷氨酸脱氢酶 来源于牛肝脏

Type III, lyophilized powder, ≥20 units/mg protein

别名:

L -GLDH, L -谷氨酸盐:NAD[P]+ 氧化还原酶(脱氨), 谷氨酸脱氢酶 来源于牛肝脏

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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-848-4
MDL number:
Specific activity:
≥20 units/mg protein
Biological source:
bovine liver
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产品名称

L-谷氨酸脱氢酶 来源于牛肝脏, Type III, lyophilized powder, ≥20 units/mg protein

form

lyophilized powder

biological source

bovine liver

type

Type III

specific activity

≥20 units/mg protein

UniProt accession no.

storage temp.

−20°C

Quality Level

Gene Information

cow ... GLUD1(281785)

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Analysis Note

双缩脲法蛋白测定

Application

L-谷氨酸脱氢酶可用于催化异柠檬酸转化为α-酮戊二酸和二氧化碳。

Biochem/physiol Actions

这种酶的哺乳动物形式,包括这种牛形式,可以使用 NADP (H) 或 NAD (H) 作为辅酶。 L -谷氨酸脱氢酶在哺乳动物代谢中起着独特角色。该酶催化的逆向反应是氨与 α-淀粉结合的唯一途径。-α 的碳原子-因此,羧酸是哺乳动物中从头合成氨基酸的唯一来源。

牛酶的特点有三组属性:
  • 具有可逆的浓度依赖性结合,产生较高分子量形式。
  • 形成紧密的酶-还原型辅酶-底物三元复合物,其解离速率调节稳态反应速率。
  • 表现出各种各样的影响,从结合的任何编号核苷酸修饰。

L-谷氨酸脱氢酶催化谷氨酸转化为 α-酮戊二酸 。

Other Notes

在25℃、pH 7.3、铵离子存在的条件下,一单位酶每分钟可将1.0 μmole α-酮戊二酸还原成 L-谷氨酸。

Packaging

包装尺寸基于蛋白质含量

Physical form

含有柠檬酸盐和磷酸钾缓冲盐。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Roy M Daniel et al.
The Biochemical journal, 425(2), 353-360 (2009-10-24)
Experimental data show that the effect of temperature on enzymes cannot be adequately explained in terms of a two-state model based on increases in activity and denaturation. The Equilibrium Model provides a quantitative explanation of enzyme thermal behaviour under reaction
Laszlo Tretter et al.
Journal of neurochemistry, 83(4), 855-862 (2002-11-08)
Previously we have reported that oxidative stress induced by hydrogen peroxide exacerbates the effect of an Na+ load in isolated nerve terminals, with a consequence of an ATP depletion, [Ca2+]i and [Na+]i deregulation, and collapse of mitochondrial membrane potential. In
Decreased carbohydrate metabolism enzyme activities in the glaucomatous trabecular meshwork
Junk AK, Goel M, Mundorf T, Rockwood EJ, Bhattacharya SK
Molecular Vision, 10, 1286-1291 (2010)
S M Kuo et al.
Biology of the neonate, 43(1-2), 23-32 (1983-01-01)
The tissue distribution, the subcellular distribution in liver, and the developmental patterns of cysteine:alpha-ketoglutarate aminotransferase (CAT) and 3-mercaptopyruvate sulfurtransferase (MPST) activities were determined in rats of the Sprague-Dawley strain. CAT activity was highest in heart and liver, whereas MPST activity
James K Kranz et al.
Methods in molecular biology (Clifton, N.J.), 796, 3-17 (2011-11-05)
Thermodynamic principles of cooperativity and allostery have long been used as a starting point to begin understanding the interplay between ligand binding events. Understanding the nature of allosteric effects requires an experimental technique that can be used to quantify ligand

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