packaging
pack of 300 mL
manufacturer/tradename
Cytiva 17-0756-04
storage condition
(20% ethanol)
parameter
<100 cm/hr flow rate (sample loading), 100-300 cm/hr flow rate (wash and elution)
technique(s)
liquid chromatography (LC): suitable
matrix
4% agarose
matrix active group
Glutathione
average diameter
90 μm
cleaning in place
4-13
working range
4-13
capacity
>25 mg binding capacity (recombinant GST / ml)
separation technique
affinity
shipped in
ambient
storage temp.
2-30°C
General description
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
Features and Benefits
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
此项目有
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GE17-0756-04 | 04061837620676 |