包装
pack of 300 mL
制造商/商品名称
Cytiva 17-0756-04
储存条件
(20% ethanol)
参数
<100 cm/hr flow rate (sample loading)
100-300 cm/hr flow rate (wash and elution)
技术
liquid chromatography (LC): suitable
基质
4% agarose
基质活性基团
Glutathione
平均直径
90 μm
cleaning in place
4-13
工作范围
4-13
容量
>25 mg binding capacity (recombinant GST / ml)
分离技术
affinity
运输
ambient
储存温度
2-30°C
一般描述
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
特点和优势
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
制备说明
分析说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
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