产品名称
Superdex® 200 Increase 3.2/300, column L × I.D. 30 cm × 3.2 mm, 8.6 μm particle size
material
glass column
titanium frit
product line
Superdex®
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 1.3 MDa (exclusion limit)
protein Mr 10-600 kDa (fractionation range)
Mp 1-100 kDa (dextrans fractionation range)
packaging
1 ea of
storage condition
(20% ethanol )
parameter
0.075 mL/min flow rate
0.15 ml/min max. flow rate
2 Mpa pressure drop required
4-50 μL sample volume
technique(s)
liquid chromatography (LC): suitable
bed volume
~2.4 mL
column L × I.D.
30 cm × 3.2 mm
matrix
agarose-dextran composite
matrix active group
dextran-agarose
particle size
8.6 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
Application
Features and Benefits
- Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
- Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
- High flow rates: rigid beads give excellent pressure/flow properties.
General description
Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.
Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.
Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.
This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows how to perform a purification of His-tagged membrane proteins.
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
实验方案
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.
Cytiva的Superdex是由葡聚糖和琼脂糖的复合基础基质组成的SEC介质。本页面介绍如何使用superdex执行分离任务。
相关内容
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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