H8509
β-羟丁酸脱氢酶 来源于球形红假单胞菌
Type V, lyophilized powder, 250-750 units/mg protein
别名:
(R)-3-羟基丁酸:NAD+ 氧化还原酶, 3-HBDH
类型
Type V
表单
lyophilized powder
比活
250-750 units/mg protein
纯化方式
chromatography
异质活性
lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%
储存温度
−20°C
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应用
适用于通过 Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974) 中介绍方法的测定乙酰乙酸酯和 D(-)-3-羟基丁酸酯。
生化/生理作用
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
外形
含 Tris 缓冲盐的冻干粉
其他说明
One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
Takashi Ide
Bioscience, biotechnology, and biochemistry, 74(8), 1578-1582 (2010-08-12)
An enzymatic-HPLC method to analyze the serum concentration of D-3-hydroxybutyric acid was developed. A deproteinized sample of rat serum was treated with 3-hydroxybutyrate dehydrogenase in the presence of NAD, and was analyzed by reversed-phase HPLC to separate and quantify NADH
Tina Hölscher et al.
Applied and environmental microbiology, 76(16), 5585-5591 (2010-06-29)
In this study, a methylotrophic bacterium, Methylobacterium rhodesianum MB 126, was used for the production of the chiral compound (R)-3-hydroxybutyrate (R-3HB) from methanol. R-3HB is formed during intracellular degradation of the storage polymer (R)-3-polyhydroxybutyrate (PHB). Since the monomer R-3HB does
C García de María et al.
Talanta, 80(3), 1436-1440 (2009-12-17)
A new kinetic method is proposed for the simultaneous determination of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) based on the different rate of the 3-hydroxybutyrate dehydrogenase-catalysed reactions of these compounds with coenzyme NAD(+). A flow injection system with two reactors of
W Hugo Ramos-Vera et al.
Journal of bacteriology, 193(5), 1201-1211 (2010-12-21)
Two autotrophic carbon fixation cycles have been identified in Crenarchaeota. The dicarboxylate/4-hydroxybutyrate cycle functions in anaerobic or microaerobic autotrophic members of the Thermoproteales and Desulfurococcales. The 3-hydroxypropionate/4-hydroxybutyrate cycle occurs in aerobic autotrophic Sulfolobales; a similar cycle may operate in autotrophic
Caiping Mao et al.
Drug and chemical toxicology, 35(2), 127-133 (2011-08-19)
To investigate whether folic acid would have toxic effects on fetal cardiac, hepatic, and renal functions, this was the first in utero fetal study testing acute effects of folic acid at the last third of gestation. Folic acid (5 mg/day) or
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