Anti-hBRM/hSNF2α (LG-14) antibody produced in rabbit

synthetic peptide corresponding to amino acids 1537-1550 of human BRM conjugated to KLH through an N-terminal added cysteine.

Anti-hBRM/hSNF2a (KR-17) antibody produced in rabbit is suitable for:

• indirect immunofluorescence: 1μg/mL using paraformaldehyde/Triton fixed hBrm-transfected 293T cell line
• microarray
• western blot at a concentration of 0.15μg/mL using nuclear extracts of the 293T cell line

Anti-hBrm/hSNF2α (LG-14) recognizes hBrm (230 kDa).

Chromatin remodeling complexes from various organisms, structurally contain related catalytic subunits, but differ in the way in which they manipulate chromatin. Three families of complexes have been described the SWI/SNF family, ISWI family, and Mi-2 family. The SWI/SNF family of ATP-dependent remodeling complexes was identified in yeast, drosophila, and human. It causes nucleosomes to change structure and/or position in order to allow transcriptional activators to gain access to their target sites. In humans, two conserved ATPase subunits have been identified as hBrm (also designated hSNF2α) and Brg1 (also designated as SNF2β). Components of the hSWI/SNF complexes have been implicated in a range of cellular events including gene activation, regulation of cell growth, and development. Brg1 and hBrm enhances transcriptional activation by glucocorticoid receptors. The remodeling complexes were traditionally associated with transcriptional activation. However, SWI/SNF has been found associated with repressor complexes, such as HDAC (histone deacetylase) and Rb (retinoblastoma) in a complex that leads to cell cycle arrest, suggesting that they are associated with transcriptional repression.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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