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线性分子式:
HOC6H4CONHNH2
化学文摘社编号:
分子量:
152.15
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
226-326-5
MDL number:
Beilstein/REAXYS Number:
473052
产品名称
4-羟基苯肼, ≥97%
InChI key
ZMZGIVVRBMFZSG-UHFFFAOYSA-N
InChI
1S/C7H8N2O2/c8-9-7(11)5-1-3-6(10)4-2-5/h1-4,10H,8H2,(H,9,11)
SMILES string
NNC(=O)c1ccc(O)cc1
biological source
synthetic
assay
≥97%
form
powder
mp
264-266 °C (dec.) (lit.)
solubility
acetic acid: water (1:1): 25 mg/mL, clear, colorless to faintly yellow
fluorescence
λex 360 nm; λem 425 nm (reacted with 5-hydroxymethyl-furaldehydezinc acetate)
Quality Level
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Application
4-羟基苯甲酰肼可用于:
- 测定淀粉消化中麦芽糖的浓度
- 分析含食物水解产物的果聚糖中的糖
- 比色测定,预估加工饲料中的还原糖
Biochem/physiol Actions
4-羟基苯甲酰肼(PAHBAH)用于分析α-淀粉酶和葡糖淀粉酶活性。基于PAHBAH的分析方法可非特异性地比色测定(415nm)样品中存在的游离还原糖基。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Dongling Qiao et al.
Carbohydrate polymers, 144, 271-281 (2016-04-17)
Combined analytical techniques were used to explore the effects of alkali treatment on the multi-scale structure and digestion behavior of starches with different amylose/amylopectin ratios. Alkali treatment disrupted the amorphous matrix, and partial lamellae and crystallites, which weakened starch molecular
Glucoamylase (exo-1, 4-alpha-d-glucan glucanohydrolase, EC 3.2. 1.3) is the major starch-degrading enzyme secreted by the phytopathogenic fungus Colletotrichum gloeosporioides
Krause DR, et al
Microbiology, 137(10), 2463-2468 (1991)
Efficacy of reducing sugar and phenol-sulfuric acid assays for analysis of soluble carbohydrates in feedstuffs
Hall, Mary Beth
Anim. Feed Sci. Technol., 185(1-2), 94-100 (2013)
Measurement of total fructan in foods by enzymatic/spectrophotometric method: collaborative study
McCleary BV, et al.
Journal of AOAC (Association of Official Analytical Chemists) International, 83(2), 356-364 (2000)
Extrusion induced low-order starch matrices: Enzymic hydrolysis and structure
Zhang B, et al.
Carbohydrate Polymers, 134(10), 485-496 (2015)
实验方案
Enzymatic Assay of Invertase
在测定转化酶活性时,使用了一种在410nm处的分光光度终点反应法进行测定。在55 ºC、pH 4.5条件下,一单位的转化酶每分钟可将1.0 μmole的蔗糖水解为转化糖。
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