重组
expressed in E. coli
描述
For cleavage of fusion proteins containing LeuGluValLeuPheGln/GlyPro sequence
方案
≥99% (SDS-PAGE)
表单
liquid
比活
≥1 units/μg
分子量
47 kDa
储存温度
−20°C
一般描述
HRV3C Protease is a recombinant restriction-grade cysteine protease. It folds into two topologically alike six-stranded β barrels. However, β barrels are different in length and individual position as well as in loops connecting elements of secondary structure. The protease is distinguishable from others by the fact that it has a cysteine nucleophile but with a chymotrypsin-like serine protease folding.
应用
HRV3C Protease has a therapeutic implication because of its unique protein structure. It may be used for the biochemical and structural characterization conducted on HRV 3C protease along with the development of 3C protease inhibitors.
Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro. Supplied as a 47 kDa protein with both GST and Histidine tags for easy removal by His-Select or Glutathione agarose along with the cleaved tag.
生化/生理作用
Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro. It is supplied as a 47 kDa protein with both GST and Histidine tags for easy removal by His-Select or Glutathione agarose along with the cleaved tag. HRV3C Protease is capable of cleaving small peptides with the sequence of polyprotein processing sites. It cleaves after the glutamine residue. HRV cleavage site generally contains Gln/Gly scissile bond.
外形
Supplied as a solution in 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM TCEP and 50% glycerol
制备说明
It is recommended to use HRV3C Protease at a protease-to-target protein ratio of 1:100 (w/w) or 1 unit of HRV3C Protease to 100 μg of target protein in a buffer suitable for the target protein at 4 °C overnight, with the target protein concentration at 1-2 mg/ml. In most cases, target proteins are completely cleaved with a protease to target protein ratio of 1:50 to 1:400, or 1 unit HRV3C Protease to 50-400 μg of target protein. The efficiency of cleavage may vary due to the sequences around the cleavage site, conformation and the solubility of the target protein. Due to its high specificity, more HRV3C Protease (at 1:10 ratio) or longer cleavage time at higher temperature (37 °C) can be used to achieve high cleavage efficiency without non-specific cleavage of target proteins.
其他说明
1 unit of HRV3C protease cleaves >95% of 100 μg of control target protein at 4 deg C for 16 hours.
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
Q May Wanga et al.
Current protein & peptide science, 8(1), 19-27 (2007-02-20)
As the major cause of the common cold in children and adults, human rhinoviruses (HRVs) are a group of small single-stranded positive-sense RNA viruses. HRVs translate their genetic information into a polyprotein precursor that is mainly processed by a virally
M G Cordingley et al.
The Journal of biological chemistry, 265(16), 9062-9065 (1990-06-05)
A series of synthetic peptides representing authentic proteolytic cleavage sites of human rhinovirus type 14 were assayed as substrates for purified 3C protease. Competition cleavage assays were employed to determine the relative specificity constants (Kcat/Km) for substrates with sequences related
D A Matthews et al.
Cell, 77(5), 761-771 (1994-06-03)
The structure of human rhinovirus-14 3C protease (3Cpro) has been determined at 2.3 A resolution and refined to an R factor of 0.22. This cysteine protease folds into two topologically equivalent six-stranded beta barrels and in this sense is similar
Adam Dyer et al.
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