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Merck
CN

HLP601

Sigma-Aldrich

Normal Human Sinusoidal Endothelial Cells; passage 1

Single-Donor Cryopreserved Sinusoidal Endothelial cells isolated from human livers, high viability, and characterized by cell specific markers

别名:

Homogenous population isolated from whole human livers

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UNSPSC代码:
41106509
NACRES:
NA.81

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生物来源

human liver

表单

frozen liquid

包装

pkg of 1 vial (contains ≥0.5 million cells)

技术

cell culture | mammalian: suitable

颜色

cloudy

储存温度

-140 to -196°C

一般描述

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Human hepatic Sinusoidal Endothelial cells (HSECs) are specialized endothelial cells in the liver that participate in the receptor-mediated clearance of endotoxin, bacteria, and other compounds. HSECs are also known to regulate inflammation, leukocyte recruitment, and host-immune responses to invading pathogens. Human HSECs are a valuable tool for the study of liver physiology and pathophysiology in vitro.

应用

Human hepatic sinusoidal endothelial cells are ideal in studies of: chronic liver injury, toxicity, immune response, portal vein fibrosis, angiogenesis, liver regeneration, stellate cell activation and more. We offer matched sets of sinusoidal endothelial cells from same donor as Hepatocytes, which can be used for co-culture systems to better simulate the complex microenvironment of the liver and gain insights into various liver-related diseases and conditions.

特点和优势

Our collection of hepatic Sinusoidal Endothelial cells is isolated through collagenase digestion and selective cell culture media. Our frozen HSECs are cryopreserved at the end of the primary culture. Each lot undergoes testing for specific cell markers and comes with a guarantee of ≥70% post-thaw viability. In addition, all cells have been tested for the absence of HIV, HBV, HCV, Syphillis and microbial contaminants (fungi, bacteria, and mycoplasma).

免责声明

Each donor is tested and found non-reactive to viral DNA from HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C. However, no known test can offer complete assurance that the viruses that cause HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C are not present. Proper precautions and biological containment should be taken when handling cells of human origin, due to their potential biohazardous nature. All human based products should be handled at a BSL-2 (Biosafety Level 2) or higher.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Frequently Asked Questions (FAQs) about Hepatocytes and Other Liver Cells

Explore answers to frequently asked questions (FAQs) about culturing primary human hepatocytes and other liver cells including Stellate cells, Kupffer cells, Sinusoidal Endothelial cells, and more.

实验方案

Primary Human Hepatic Kupffer Cell Culture Protocol

Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.

Primary Human Hepatic Sinusoidal Endothelial Cells Culture Protocol

Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.

相关内容

Pre-tested, Cryopreserved, and Ready-to-Screen Human Primary Liver Cells

Explore our primary liver cells collection, including primary human Hepatocytes (PHH), spheroid-qualified Hepatocytes, Kupffer cells, Stellate cells, Cholangiocytes, and Sinusoidal Endothelial cells.

Primary Human Hepatic Stellate Cells Culture Protocol

Discover our collection of primary human hepatic stellate cells and protocol for thawing, plating, and growing stellate cells. Find our stellate culture maintenance media formulation.

Primary Human Intrahepatic Biliary Epithelial Cells (Cholangiocytes) Culture Protocol

Discover our primary human intrahepatic biliary epithelial cells, also known as cholangiocytes. Explore our plating and maintenance media formulations and protocol for culture.

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