biological source
human
recombinant
expressed in E. coli
assay
>97% (SDS-PAGE)
form
lyophilized powder
potency
30-60 ng/mL ED50
mol wt
8.5 kDa
storage condition
avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CCL1(6346)
Biochem/physiol Actions
I-309, a chemoattractant for monocytes but not neutrophils, is an approximately 8.5 kDa polypeptide of 73 amino acids. Human I-309 is thought to be a homolog of the mouse TCA3, and shows 42% amino acid homology to mouse TCA3.
I-309, a glycoprotein, is a chemoattractant for monocytes. It is secreted by monocytes, activated macrophages and T lymphocytes. Presence of I-309 in lymph node lymphatic sinuses controls metastases into the lymph nodes. It suppresses CCR8 (C-C motif chemokine receptor 8)-induced HIV (human immunodeficiency virus) infection and also participates in angiogenesis. I-309 is also referred to as CCL1 (C-C motif chemokine ligand 1).
Physical form
Lyophilized from 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.
Analysis Note
Bioactivity measured by ability of I-309 to chemoattract hCCR8 transfected BaF/3 cells.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Eye Irrit. 2
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Suvendu Das et al.
The Journal of experimental medicine, 210(8), 1509-1528 (2013-07-24)
Lymphatic vessels are thought to contribute to metastasis primarily by serving as a transportation system. It is widely believed that tumor cells enter lymph nodes passively by the flow of lymph. We demonstrate that lymph node lymphatic sinuses control tumor
Rita Crinelli et al.
Antioxidants (Basel, Switzerland), 10(2) (2021-02-04)
I-152 combines two pro-glutathione (GSH) molecules, namely N-acetyl-cysteine (NAC) and cysteamine (MEA), to improve their potency. The co-drug efficiently increases/replenishes GSH levels in vitro and in vivo; little is known about its mechanism of action. Here we demonstrate that I-152
M D Miller et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(7), 2950-2954 (1992-04-01)
The human cytokine I-309 is a small glycoprotein secreted by activated T lymphocytes and structurally related to a number of inflammatory cytokines. To investigate the biological activities of I-309 protein, we produced a stable Chinese hamster ovary cell transfectant, CDI.10