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Merck
CN

I9392

异戊醇

BioReagent, ≥98.5%, Molecular Biology

别名:

3-甲基-1-丁醇, 3-甲基丁醇, 异戊基醇

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关于此项目

线性分子式:
(CH3)2CHCH2CH2OH
化学文摘社编号:
分子量:
88.15
Beilstein:
1718835
EC 号:
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.52
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等级

Molecular Biology

质量水平

产品线

BioReagent

方案

≥98.5%

表单

liquid

技术

RNA extraction: suitable

pH值(酸碱度)

5.6 (20 °C, 25 g/L)

密度

0.809 g/mL at 20 °C (lit.)

适用性

suitable for nucleic acid purification

储存温度

room temp

SMILES字符串

CC(C)CCO

InChI

1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3

InChI key

PHTQWCKDNZKARW-UHFFFAOYSA-N

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一般描述

3-甲基丁醇(异戊醇)是一种透明、无色的醇,常用作有机溶剂。也可用于酚-氯仿核酸提取和纯化方案中,在此过程中抑制 RNase 活性,减少起泡。异戊醇也可用于从 CsCl 梯度超速离心纯化的 DNA 中去除溴化乙锭。

应用

3-甲基丁醇已被用作苯酚/氯仿/异戊醇溶液的成分,用于从血液以及吸收基质法医样品和生物膜中提取DNA。
3-甲基丁醇(异戊醇)与苯酚和氯仿联合用于提取 RNA。它被用于使用光敏腺苷分子合成低聚核苷酸-RNA。

警示用语:

Danger

危险分类

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

3 - Flammable liquids

WGK

WGK 1

闪点(°F)

110.3 °F - closed cup

闪点(°C)

43.5 °C - closed cup

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Section 1 Isolation of nucleic acids
Biofouling Methods, 88-88 (2014)
DNA extraction from blood and forensic samples
Guidelines for Molecular Analysis in Archive Tissues, 45-54 (2011)
Steven G Chaulk et al.
Nature protocols, 2(5), 1052-1058 (2007-06-05)
This protocol describes a general method for the preparation of RNAs in which the reactivity or hydrogen-bonding properties of the molecule are modified in a photoreversible fashion by use of a caging strategy. A single caged adenosine, modified at the
Kazushi Yoshida et al.
Nature communications, 3, 739-739 (2012-03-15)
The same odorant can induce attractive or repulsive responses depending on its concentration in various animals including humans. However, little is understood about the neuronal basis of this behavioural phenomenon. Here we show that Caenorhabditis elegans avoids high concentrations of
Mary J Dunlop et al.
Molecular systems biology, 7, 487-487 (2011-05-11)
Many compounds being considered as candidates for advanced biofuels are toxic to microorganisms. This introduces an undesirable trade-off when engineering metabolic pathways for biofuel production because the engineered microbes must balance production against survival. Cellular export systems, such as efflux

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