K1502
α-酮戊二酸脱氢酶 来源于猪心脏
buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)
别名:
多酶2-氧戊二酸脱氢酶配合物
一般描述
研究领域：Neuroscience
α-酮戊二酸脱氢酶（α-KGDH）是位于线粒体的一种多酶复合体。这种复合酶由多个单位的硫胺素焦磷酸依赖性脱氢酶(E1)、二氢硫辛酰脱氢酶(E3)和二氢硫辛酰转琥珀酰酶(E2)组成。
α-酮戊二酸脱氢酶（α-KGDH）是位于线粒体的一种多酶复合体。这种复合酶由多个单位的硫胺素焦磷酸依赖性脱氢酶(E1)、二氢硫辛酰脱氢酶(E3)和二氢硫辛酰转琥珀酰酶(E2)组成。
应用
α猪心α-酮戊二酸脱氢酶已用于：
- 研究过氧亚硝酸盐处理细胞中谷胱甘肽(GSH)的反硝化作用
- 通过Spectramax M5微孔板分光荧光计检测其在心肌线粒体中的活性
- 作为阳性对照，通过Spectramax GEMINI EM荧光微孔板读板机评估其在小鼠神经元中的活性
生化/生理作用
αα-酮戊二酸脱氢酶(α-KGDH)是生物能量学过程的关键酶，是Krebs循环或三羧酸(TCA)循环代谢流的调控物。它通过还原型烟酰胺腺嘌呤二核苷酸(NADH)的释放，催化α-酮戊二酸氧化脱羧为琥珀酰-CoA。这是TCA循环中的限速反应。该反应为呼吸链提供电子，需要硫胺素焦磷酸作为辅因子。反应速率由NAD（烟酰胺腺嘌呤二核苷酸）还原决定。αα-KGDH的最佳pH值范围为6.6-7.4。该酶会被氧化应激抑制并导致代谢缺乏。但α-KGDH又能产生活性氧(ROS)导致氧化应激。α-KGDH缺陷或浓度不够会导致好几种神经退行性疾病，比如阿尔茨海默病。
α-酮戊二酸脱氢酶对肿瘤微环境改变的反应最灵敏，与癌症的适应性代谢反应有关。抑制α-酮戊二酸脱氢酶可对抗乳腺癌相关的肺转移。
外形
以 50％ 甘油溶液的形式提供，含有 9mg 每毫升的牛血清白蛋白、30％ 蔗糖、1.5 mM EDTA、1.5 mM EGTA、1.5 mM 2-巯基乙醇、0.3％ TRITON™ X-100、0.003％ 叠氮化钠和 15 mM 磷酸钾，pH 6.8。
分析说明
可能含有痕量的聚乙二醇。
其他说明
在 30 °C、pH 7.4、饱和水平的辅酶 A 存在的条件下，一单位每分钟可将 1.0 μ 摩尔的 β-NAD 转化成 β-NADH。
法律信息
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
危险声明
预防措施声明
危险分类
Aquatic Chronic 3
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
含少量动物源组分生物产品
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