等级
Molecular Biology
方案
>99% (SDS-PAGE)
表单
buffered aqueous solution
比活
3,000,000 U/mg
浓度
3,000,000 U/mL
运输
dry ice
储存温度
−20°C
一般描述
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA.
应用
Suitable for applications requiring high cohesive end ligation efficiency (TALE).
特点和优势
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
外形
Supplied in 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.
其他说明
1 unit is defined as the amount of T7 DNA Ligase required to ligate 50% of 100 ng DNA fragments with cohesive termini in 30 minutes at 23° C.
Source of protein: A recombinant E. coli strain carrying the T7 DNA Ligase gene.
Supplied with:KEM0046B (2X Rapid Ligation Buffer)
Unit size: 900,000 U
储存分类代码
10 - Combustible liquids
法规信息
新产品
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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