grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
3,000,000 U/mg
concentration
3,000,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA.
Application
Suitable for applications requiring high cohesive end ligation efficiency (TALE).
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.
Other Notes
1 unit is defined as the amount of T7 DNA Ligase required to ligate 50% of 100 ng DNA fragments with cohesive termini in 30 minutes at 23° C.
Source of protein: A recombinant E. coli strain carrying the T7 DNA Ligase gene.
Supplied with:KEM0046B (2X Rapid Ligation Buffer)
Unit size: 900,000 U
存储类别
10 - Combustible liquids
法规信息
新产品
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Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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