等级
Molecular Biology
方案
>99% (SDS-PAGE)
表单
buffered aqueous solution
比活
5,000 U/mg
浓度
5,000 U/mL
运输
dry ice
储存温度
−20°C
一般描述
Klenow Fragment is a mesophilic DNA polymerase derived from the E.coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3′ → 5′) nuclease activities, and, in the absence of the holoenzyme′s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene.
应用
Suitable for:
- DNA blunting by fill-in of 5′ overhang
- Second strand cDNA synthesis
- Sequencing
- Site-specific mutagenesis
特点和优势
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
外形
Supplied in 100 mM KPO4, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 7.4 @ 25° C.
其他说明
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37° C.
Source of protein: A recombinant E. coli strain carrying the Klenow Fragment gene.
Supplied with:KEM0042B (10X Blue Buffer)
Unit size: 2,500 U
储存分类代码
10 - Combustible liquids
法规信息
新产品
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持