grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
5,000 U/mg
concentration
5,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
Klenow Fragment is a mesophilic DNA polymerase derived from the E.coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3′ → 5′) nuclease activities, and, in the absence of the holoenzyme′s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene.
Application
Suitable for:
- DNA blunting by fill-in of 5′ overhang
- Second strand cDNA synthesis
- Sequencing
- Site-specific mutagenesis
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 100 mM KPO4, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 7.4 @ 25° C.
Other Notes
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37° C.
Source of protein: A recombinant E. coli strain carrying the Klenow Fragment gene.
Supplied with:KEM0042B (10X Blue Buffer)
Unit size: 2,500 U
存储类别
10 - Combustible liquids
法规信息
新产品
此项目有
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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